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Protein secretion of VEGF and sFlt1 from first (FTB) and third (TTB) trimester trophoblast. a Quantification of VEGF in CM of FTB versus TTB measured by dot blot analysis. b Quantification of sFlt1 in CM of FTB versus TTB measured by ELISA. Data are given as mean ± SEM. Statistical analysis used the mean of the triplicate of the individual biological replicates. n (dot blot) = 4, n (ELISA) = 8
