Figure 2.

Flight activity rhythm of a single mated An. stephensi female mosquito continuously monitored for eleven days in a 12:12 light:dark cycle with 1 h-long dawn and dusk transitions, followed by a further eight days in constant dark conditions. Note, under the entraining conditions of a light:dark cycle, flight activity begins at approximately the same time each day. Under constant dark:dark conditions (where there are no light cues to entrain the clock), flight activity maintains a rhythm driven by its non-entrained, free running circadian clock. In the An. stephensi mosquito, the non-entrained clock free runs at a period longer than 24 h as evidenced by flight activity beginning slightly later each day. Recording was performed using a locomotor activity monitor (L.A.M.) from TriKinetics, Inc. (Waltham, MA, USA) at 26 °C and 60% relative humidity. See Rund et al. 2012 for the methods [67].