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Primary antibody concentrations for Western Blot analysis of phosphorylated glucocorticoid receptor (P-GR), total glucocorticoid receptor (GR), FKBP51, and steroid receptor co-activator (SRC)-1 in the paraventricular nucleus (PVN), central amygdala (CeA) and ventral hippocampus (VH).
| PVN | CeA | VH | |
|---|---|---|---|
| pGR (Ser 232) | 1:500 | 1:1000 | 1:1000 |
| GR | 1:1000 | 1:1000 | 1:1000 |
| FKBP51 | 1:20,000 | 1:10,000 | 1:20,000 |
| SRC-1 | 1:1000 | 1:1000 | 1:1000 |
