Skip to main content
. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Neuron. 2016 Jun 16;91(1):25–33. doi: 10.1016/j.neuron.2016.05.025

Figure 4. PAK is required for fasting- and AMPK-stimulated synaptic plasticity.

Figure 4

(A–C) Schematics of dominant negative AAV-DIO-DN-PAK (A) and stereotaxic injection (B), and immunofluorescence of EGFP (C) in Agrp-IRES-Cre mice.

(D–K) Examples and summary of dendritic spines (D and E), mEPSCs (F–H), and firing properties (I–K) in 24-hr fasted Npy-hrGFP control and AAV-DIO-DN-PAK virus-injected Agrp-IRES-Cre mice.

(L–P) Schematic of AAV-DIO-CA-AMPK and AAV-DIO-DN-PAK viral injection (L), immunofluorescence (M), and example and summery of mEPSCs (N–P).

(Q and R) Body weight (Q) and food eaten following 24-hr fasting (R) from mice bilaterally injected with AAV-DIO-DN-PAK (n=8).

Data are mean ± SEM (n=10 neurons from 3 mice per group in E, G, H, J, K, O, and P) and * indicates p<0.05 with unpaired two-tailed student’s t-test.