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. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Structure. 2016 Jun 16;24(7):1044–1056. doi: 10.1016/j.str.2016.04.020

Table 2.

Catalytic efficiency for hNaa60 WT and mutants

Enzyme Kcat/Km (μM−1 min−1) Wild-type efficiency (%)
WT 2. 27 ×10−2 100
P35A 0.36 ×10−2 16.5
I36A 0.36 ×10−2 16.6
P35A/I36A/I167A ND <10
Y38A ND <10
D81A 3.11 ×10−2 136.9
I84A 4.55 ×10−2 200.4
Y97F ND <10
L140A 1.26 ×10−2 56.0
Y164A 3.21 ×10−2 141.7
Y164F 3.51 ×10−2 154.9
Y165F 0.54 ×10−2 24.4
I167A 0.74 ×10−2 33.3