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. Author manuscript; available in PMC: 2016 Dec 30.
Published in final edited form as: Nature. 2016 Jun 30;534(7609):710–713. doi: 10.1038/nature18302

Extended Data Figure 7. Rescue of UPRmt-induced mitochondrial pre-RNA processing by MRPP3 overexpression.

Extended Data Figure 7

a, Western Blot analysis of MRPP3 levels upon DMSO or GTPP treatment in the context of wild-type or MRPP3-overexpressing (o/e) cells. Quantifications of the MRPP3 bands are shown in blue (quantified with Fiji of digitally acquired images). b, Quantitative PCR analysis of non-processed mitochondrial pre-RNA levels at the tRNAMet and tRNALys cut sites in wild-type cells or cells overexpressing MRPP3. Shown are mean values ±s.d. (n=3 biological replicates). c, Quantitative PCR analysis of non-processed mitochondrial pre-RNA levels at the tRNAMet and tRNALys cut sites in wild-type cells or cells overexpressing MRPP3 upon GTPP treatment. Shown are mean values ±s.d. (n=3 biological replicates).