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. 2015 Nov 2;7(13):15507–15524. doi: 10.18632/oncotarget.6266

Figure 3. Functional CSC properties in RCC41-PDX-1/CD132+, unsorted RCC-41-PDX2 cells, RCC-41-PDX-2/CD133−, and RCC41-PDX-2/CD133+ subsets.

Figure 3

(A) The aldehyde dehydrogenase (ALDH) activity in sorted RCC-41-PDX-1/CD132+, unsorted RCC-41-PDX2, RCC-41-PDX-2/CD133+ and RCC-41-PDX-2/CD133− was measured by flow cytometry (10 000 events). ALDH activity was measured in the absence and presence of the specific ALDH inhibitor DEAB following manufacturer's instructions. One experiment representative of three is shown. (B, C) Spheroïd formation following limiting dilution assay of the indicated cells. Data represent mean values of five replicates for two experiments; error bars correspond to 95% confidence intervals. (D) Clonogenic activity at limiting dilution: sorted RCC-41-PDX-1/CD132+, RCC-41-PDX-2/CD133+ and RCC-41-PDX-2/CD133− were plated in the absence of serum at a density of 1 to 10 cells per well in 24-well plates containing DMEM-LG medium. After 2 weeks, each well was examined under a light microscope, and the total number of wells with colonies was determined. Data represent mean values of three replicates for three experiments; error bars correspond to 95% confidence intervals.