Figure 2. The store-operated Ca2+ entry decreased after TGF-β treatment in MDA-MB-231 cells.

A. The cytosolic Ca²⁺ concentration was measured in Fura 2-AM-loaded MDA-MB-231 cells. The dynamic intracellular Ca²⁺ concentration was shown as the ratio of F340/F380. B. The quantitative analysis of Ca²⁺ content induced by TG. C. The quantitative analysis of Ca²⁺ influx. For this assay, three individual experiments (about 10 cells each time, totally more than 30 cells for each group) were performed. Data were expressed as means ± SE. Significance was assessed using one-way ANOVA with Bonferroni's multiple comparisons post-tests, *P < 0.05.