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. 2016 Feb 23;7(13):16866–16878. doi: 10.18632/oncotarget.7619

Figure 3. The specific SOCE inhibitor SKF96365 impaired TGF-β-induced suppression of cell proliferation and cell cycle arrest in MDA-MB-231 cells.

Figure 3

A-B. Cells were cultured with DMEM supplemented with 10% FBS containing TGF-β (5 ng/ml), SKF96365 (1.5 μM), TGF-β (5 ng/ml) plus SKF96365 (1.5 μM) respectively for 3 and 4 days. The cell proliferation was measured by MTT assay. *P<0.05 VS. Control group without TGF-β treatment. n.s (not significant) VS. SKF 1.5 μM treatment group without TGF-β treatment. C. The cell cycle distribution after 3 days' culture was detected by flow cytometry, and the percentage of the cells at G0/G1, S and G2/M phase with various treatments was analyzed. D. The statistical analysis of cell cycle distribution at G0/G1, S and G2/M phases. For this assay, bar graphs showed means ± SE for at least three independent experiments. Significance was assessed using student's t-test, *P < 0.05.