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. 2016 Feb 24;7(13):16962–16974. doi: 10.18632/oncotarget.7655

Figure 7. BPAP causes changes in cell metabolism and mitochondria.

Figure 7

A. Metabolomics Phenotype Arrays (sugar sources) were performed in 22Rv1 cells after treatment with vehicle or BPAP 20μM for 24h. Heatmap represents differential carbon source usage between treatments as represented by Z-score, each column represents biological replicates. B–C. BPAP reduces mitochondria size and intensity of signal after 24 hr of treatment in 22Rv1 cells. Images were taken with 100x objective on a DeltaVision deconvolution microscope. Size and intensity of mitochondria were measured via automated image analysis routines. D. 22Rv1 were treated with BPAP 10–40μM for 24hrs and then stained with JC-1 to measure mitochondrial potential. Graph shows red/green ratio that represents mitochondria functionality. 50μM CCCP was used as positive control.