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. 2016 Mar 6;7(13):17144–17161. doi: 10.18632/oncotarget.7942

Figure 3. Effects of siMMP-13 knockdown on the EMT status in invasive oral cancer cells.

Figure 3

(A) Total cell lysates were prepared from untreated or siMMP-13-treated cells. The cell lysates were immunoblotted with antibodies against EMT transcription factors, Twist, p-Src, Snail1, SIP1, JAM-A, and vinculin. (B) Total cell lysates were prepared from untreated or siMMP-13-treated cells. The cell lysates were immunoblotted with antibodies against EMT downstream proteins, E-cadherin, and vimentin. Alpha-tubulin was considered the loading control. According to ImageQuant TL 1D gel analysis, after each value was normalized with its alpha-tubulin, the obtained values were normalized with their antibodies stained in OC3. (C) Antibodies used in the immunoblots are listed in the table.