Abstract
A commercial kit method (Technia Diagnostics) for the estimation of serum vitamin B12 claiming certain practical advantages was examined. Analytical and clinical performance were compared with a non-commercial radioisotope B12 method, previously compared to other commercial radioisotope B12 methods. The kit's analytical performance in our hands was satisfactory, although the within-batch precision and recovery of added cyanocobalamin were disappointing. Clinical performance was comparable with the non-commercial B12 method. Establishment of suitable reference ranges as a prerequisite to diagnostic use is apparent.
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Selected References
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- Dawson D. W., Delamore I. W., Fish D. I., Flaherty T. A., Gowenlock A. H., Hunt L. P., Hyde K., MacIver J. E., Thornton J. A., Waters H. M. An evaluation of commercial radioisotope methods for the determination of folate and vitamin B12. J Clin Pathol. 1980 Mar;33(3):234–242. doi: 10.1136/jcp.33.3.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
- England J. M., Linnell J. C. Problems with the serum vitamin B12 assay. Lancet. 1980 Nov 15;2(8203):1072–1074. doi: 10.1016/s0140-6736(80)92287-4. [DOI] [PubMed] [Google Scholar]
- Green R., Newmark P. A., Musso A. M., Mollin D. L. The use of chicken serum for measurement of serum vitamin B12 concentration by radioisotope dilution: discription of method and comparison with microbiological assay results. Br J Haematol. 1974 Jul;27(3):507–526. doi: 10.1111/j.1365-2141.1974.tb06816.x. [DOI] [PubMed] [Google Scholar]
- Hippe E., Olesen H. Nature of vitamin B 12 binding. 3. Thermodynamics of binding to human intrinsic factor and transcobalamins. Biochim Biophys Acta. 1971 Jul 25;243(1):83–88. [PubMed] [Google Scholar]
- Kidroni G., Grossowicz N. Nature of vitamin B 12 binding by native serum, seromucoid, and highly purified fractions from chicken serum. Biochim Biophys Acta. 1971 Nov 12;252(2):262–273. doi: 10.1016/0304-4165(71)90006-7. [DOI] [PubMed] [Google Scholar]
- Matthews D. M., Gunasegaram R., Linnell J. C. Results with radioisotopic assay of serum B12 using serum binding agent. J Clin Pathol. 1967 Sep;20(5):683–686. doi: 10.1136/jcp.20.5.683. [DOI] [PMC free article] [PubMed] [Google Scholar]