Abstract
The applicability of a hapten-antihapten sandwich technique incorporating a monoclonal antihapten bridge antibody to localisation of tissue antigen is described. The results show that the method is both versatile and selective and has the potential of being even more sensitive than currently used immunoperoxidase methods.
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- Cammisuli S., Wofsy L. Hapten-sandwich labeling. III. Bifunctional reagents for immunospecific labeling of cell surface antigens. J Immunol. 1976 Nov;117(5 Pt 1):1695–1704. [PubMed] [Google Scholar]
- DeLellis R. A., Sternberger L. A., Mann R. B., Banks P. M., Nakane P. K. Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute. Am J Clin Pathol. 1979 May;71(5):483–488. doi: 10.1093/ajcp/71.5.483. [DOI] [PubMed] [Google Scholar]
- El Etreby M. F., Mahrous A. T. Immunocytochemical technique for detection of prolactin (PRL) and growth hormone (GH) in hyperplastic and neoplastic lesions of dog prostate and mammary gland. Histochemistry. 1979;64(3):279–286. doi: 10.1007/BF00495028. [DOI] [PubMed] [Google Scholar]
- Heyderman E. Immunoperoxidase technique in histopathology: applications, methods, and controls. J Clin Pathol. 1979 Oct;32(10):971–978. doi: 10.1136/jcp.32.10.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976 Jul;6(7):511–519. doi: 10.1002/eji.1830060713. [DOI] [PubMed] [Google Scholar]
- Mason D. Y., Sammons R. Rapid preparation of peroxidase: anti-peroxidase complexes for immunocytochemical use. J Immunol Methods. 1978;20:317–324. doi: 10.1016/0022-1759(78)90267-3. [DOI] [PubMed] [Google Scholar]
- Witorsch R. J., Smith J. P. Evidence for androgen-dependent intracellular binding of prolactin in rat ventral prostate gland. Endocrinology. 1977 Sep;101(3):929–938. doi: 10.1210/endo-101-3-929. [DOI] [PubMed] [Google Scholar]