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. 1981 Sep;34(9):1010–1016. doi: 10.1136/jcp.34.9.1010

Isolation of Mallory bodies and an attempt to demonstrate cell mediated immunity to Mallory body isolate in patients with alcoholic liver disease.

C Gluud, F Hardt, J Aldershvile, P Christoffersen, H Lyon, J Nielsen
PMCID: PMC494232  PMID: 6168660

Abstract

Mallory bodies were isolated from necropsy livers from patients with alcoholic hepatitis with and without cirrhosis with a Ficoll viscosity barrier. The purity of Mallory bodies in the isolate varied between 70 and 90%, estimated by counting Mallory bodies and non-Mallory body structures in haematoxylin-eosin stained smears. Electron microscopy confirmed the presence of Mallory bodies in the isolates. The Mallory body isolate was used as antigen in the agarose leucocyte migration inhibition test in order to test the cell-mediated immunity. No significant difference in leucocyte migration was found between controls and patients with alcoholic hepatitis, alcoholic steatosis, alcoholic cirrhosis and miscellaneous liver diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldershvile J., Dietrichson O., Hardt F., Nielsen J. O., Skinhöj P. Humoral and cell-mediated immunity to hepatitis B virus antigens in acute and chronic liver disease. Scand J Gastroenterol. 1977;12(8):917–922. doi: 10.3109/00365527709181350. [DOI] [PubMed] [Google Scholar]
  2. Bock E., Rasmussen S., Moller M., Ebbesen P. Demonstration of a protein immunochemically related to glial fibrillary acidic protein in human fibroblasts in culture. FEBS Lett. 1977 Nov 15;83(2):212–216. doi: 10.1016/0014-5793(77)81007-7. [DOI] [PubMed] [Google Scholar]
  3. Bull D. M. Assessment of autoantigenic activity of hepatic tissue components. Gastroenterology. 1976 Jul;71(1):164–166. [PubMed] [Google Scholar]
  4. Franke W. W., Denk H., Schmid E., Osborn M., Weber K. Ultrastructural, biochemical, and immunologic characterization of Mallory bodies in livers of griseofulvin-treated mice. Fimbriated rods of filaments containing prekeratin-like polypeptides. Lab Invest. 1979 Feb;40(2):207–220. [PubMed] [Google Scholar]
  5. French S. W., Ihrig T. J., Norum M. L. A method of isolation of Mallory bodies in a purified fraction. Lab Invest. 1972 Mar;26(3):240–244. [PubMed] [Google Scholar]
  6. Junge U., Kehl A., Schober A., Winckler K. Immunological aspects of alcoholic hepatitis. Gastroenterology. 1978 Jul;75(1):165–166. [PubMed] [Google Scholar]
  7. Kanagasundaram N., Kakumu S., Chen T., Leevy C. M. Alcoholic hyalin antigen (AHAg) and antibody (AHAb) in alcoholic hepatitis. Gastroenterology. 1977 Dec;73(6):1368–1373. [PubMed] [Google Scholar]
  8. Kehl A., Schober A., Junge U., Winckler K. Solid-phase radioimmunoassay for detection of alcoholic hyalin antigen (AHAg) and antibody (anti-AH). Clin Exp Immunol. 1981 Jan;43(1):215–221. [PMC free article] [PubMed] [Google Scholar]
  9. Leeve C. M., Chent, Zetterman R. Alcoholic hepatitis, cirrhosis, and immunologic reactivity. Ann N Y Acad Sci. 1975 Apr 25;252:106–115. doi: 10.1111/j.1749-6632.1975.tb19147.x. [DOI] [PubMed] [Google Scholar]
  10. Leevy C. M., Chen T., Luisada-Opper A., Kanagasundaram N., Zetterman R. Liver disease of the alcoholic: role of immunologic abnormalities in pathogenesis, recognition, and treatment. Prog Liver Dis. 1976;5:516–530. [PubMed] [Google Scholar]
  11. Luisada-Opper A. V., Kanagasundaram N., Leevy C. M. Chemical nature of alcoholic hyalin. Gastroenterology. 1977 Dec;73(6):1374–1376. [PubMed] [Google Scholar]
  12. Nenci I. Identification of actin-like proteins in alcoholic hyaline by immunofluorescence. Lab Invest. 1975 Feb;32(2):257–260. [PubMed] [Google Scholar]
  13. Okamura K., Harwood T. R., Yokoo H. Isolation and amino acid analysis of Mallory bodies. Prep Biochem. 1974;4(4):331–340. doi: 10.1080/00327487408068208. [DOI] [PubMed] [Google Scholar]
  14. Okamura K., Harwood T. R., Yokoo H. Isolation and electrophoretic study on Mallory bodies from the livers of alcoholic cirrhosis. Lab Invest. 1975 Aug;33(2):193–199. [PubMed] [Google Scholar]
  15. Tinberg H. M., Regan R. J., Geier E. A., Peterson G. E., French S. W. Mallory bodies: isolation of hepatocellular hyalin and electrophoretic resolution of polypeptide components. Lab Invest. 1978 Nov;39(5):483–490. [PubMed] [Google Scholar]
  16. Triggs S. M., Mills P. R., MacSween R. N. Sensitisation to Mallory bodies (alcoholic hyalin) in alcoholic hepatitis. J Clin Pathol. 1981 Jan;34(1):21–24. doi: 10.1136/jcp.34.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yokoo H., Minick O. T., Batti F., Kent G. Morphologic variants of alcoholic hyalin. Am J Pathol. 1972 Oct;69(1):25–40. [PMC free article] [PubMed] [Google Scholar]
  18. Zetterman R. K., Leevy C. M. Immunologic reactivity and alcoholic liver disease. Bull N Y Acad Med. 1975 Apr;51(4):533–544. [PMC free article] [PubMed] [Google Scholar]
  19. Zetterman R. K., Luisada-Opper A., Leevy C. M. Alcoholic hepatitis. Cell-mediated immunological response to alcoholic hyalin. Gastroenterology. 1976 Mar;70(3):382–384. [PubMed] [Google Scholar]

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