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. 1973 Jun;36(3):389–398. doi: 10.1136/jnnp.36.3.389

Synaptogenesis in cell cultures of neurones and myotubes from chickens with muscular dystrophy

John H Peacock 1,2,1, Phillip G Nelson 1,2
PMCID: PMC494338  PMID: 4714101

Abstract

Intracellular microelectrode recordings from chick dystrophic myotubes in cell culture reveal a capability for innervation by neurones from either dystrophic or normal embryos. Neither neuronal class differentially affects the incidence of synapse formation at neuromuscular junctions (about 75%) or at neural junctions (about 85%), the PSP frequency (about 10/sec), the maximum quantal content at neuromuscular junctions (over 70), or the resting membrane potentials of either myotubes (about − 53 mV) or neurones (about − 43 mV). In each culture conditon about 20% of nerve-muscle cell pairs exhibit bidirectional electrical coupling. Dual innervation of a muscle and nerve cell from a common presynaptic source sometimes occurs and both muscle and nerve cells probably have multiple innervation. Assuming the capability for expression in culture of genetic differences between neurones from the dystrophic and normal chick, we conclude that these differences are not significant in the regulation of synapse formation in dystrophic chick nerve-muscle cell culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albuquerque E. X., Warnick J. E. Electrophysiological observations in normal and dystrophic chicken muscles. Science. 1971 Jun 18;172(3989):1260–1263. doi: 10.1126/science.172.3989.1260. [DOI] [PubMed] [Google Scholar]
  2. Askanas V., Shafiq S. A., Milhorat A. T. Normal and dystrophic chicken muscle at successive stages in tissue culture. Arch Neurol. 1971 Mar;24(3):259–265. doi: 10.1001/archneur.1971.00480330087008. [DOI] [PubMed] [Google Scholar]
  3. BORNSTEIN M. B. Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes. Lab Invest. 1958 Mar-Apr;7(2):134–137. [PubMed] [Google Scholar]
  4. Caspary E. A., Currie S., Field E. J. Sensitized lymphocytes in muscular dystrophy: evidence for a neural factor in pathogenesis. J Neurol Neurosurg Psychiatry. 1971 Jun;34(3):353–356. doi: 10.1136/jnnp.34.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DANIEL P. M., STRICH S. J. ABNORMALITIES IN THE MUSCLE SPINDLES IN DYSTROPHIA MYOTONICA. Neurology. 1964 Apr;14:310–316. doi: 10.1212/wnl.14.4.310. [DOI] [PubMed] [Google Scholar]
  6. Fambrough D. M. Acetylcholine sensitivity of muscle fiber membranes: mechanism of regulation by motoneurons. Science. 1970 Apr 17;168(3929):372–373. doi: 10.1126/science.168.3929.372. [DOI] [PubMed] [Google Scholar]
  7. Fischbach G. D. Synapse formation between dissociated nerve and muscle cells in low density cell cultures. Dev Biol. 1972 Jun;28(2):407–429. doi: 10.1016/0012-1606(72)90023-1. [DOI] [PubMed] [Google Scholar]
  8. Grampp W., Harris J. B., Thesleff S. Inhibition of denervation changes in mammalian skeletal muscle by actinomycin D. J Physiol. 1971;217 (Suppl):47P–48P. [PubMed] [Google Scholar]
  9. Guth L. "Trophic" influences of nerve on muscle. Physiol Rev. 1968 Oct;48(4):645–687. doi: 10.1152/physrev.1968.48.4.645. [DOI] [PubMed] [Google Scholar]
  10. Guth L., Samaha F. J., Albers R. W. The neural regulation of some phenotypic differences between the fiber types of mammalian skeletal muscle. Exp Neurol. 1970 Jan;26(1):126–135. doi: 10.1016/0014-4886(70)90094-4. [DOI] [PubMed] [Google Scholar]
  11. Harris J. B. The relation of trophic influences to diseases of muscle. Dev Med Child Neurol. 1971 Oct;13(5):669–671. [PubMed] [Google Scholar]
  12. Harris J. B., Wallace C., Wing J. Myelinated nerve fibre counts in the nerves of normal and dystrophic mouse muscle. J Neurol Sci. 1972 Mar;15(3):245–249. doi: 10.1016/0022-510x(72)90067-6. [DOI] [PubMed] [Google Scholar]
  13. Hartzell H. C., Fambrough D. M. Acetylcholine receptors. Distribution and extrajunctional density in rat diaphragm after denervation correlated with acetylcholine sensitivity. J Gen Physiol. 1972 Sep;60(3):248–262. doi: 10.1085/jgp.60.3.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jasmin G., Bokdawala F. Muscle transplantation in normal and dystrophic hamsters. Rev Can Biol. 1970 Jun;29(2):197–201. [PubMed] [Google Scholar]
  15. McComas A. J., Campbell M. J., Sica R. E. Electrophysiological study of dystrophia myotonica. J Neurol Neurosurg Psychiatry. 1971 Apr;34(2):132–139. [PMC free article] [PubMed] [Google Scholar]
  16. McComas A. J., Mrozek K. Denervated muscle fibres in hereditary mouse dystrophy. J Neurol Neurosurg Psychiatry. 1967 Dec;30(6):526–530. doi: 10.1136/jnnp.30.6.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McComas A. J., Sica R. E., Currie S. An electrophysiological study of Duchenne dystrophy. J Neurol Neurosurg Psychiatry. 1971 Aug;34(4):461–468. doi: 10.1136/jnnp.34.4.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mendell J. R., Engel W. K., Derrer E. C. Duchenne muscular dystrophy: functional ischemia reproduces its characteristic lesions. Science. 1971 Jun 11;172(3988):1143–1145. doi: 10.1126/science.172.3988.1143. [DOI] [PubMed] [Google Scholar]
  19. Nelson P. G., Peacock J. H., Amano T., Minna J. Electrogenesis in mouse neuroblastoma cells in vitro. J Cell Physiol. 1971 Jun;77(3):337–352. doi: 10.1002/jcp.1040770308. [DOI] [PubMed] [Google Scholar]
  20. Peacock J. H., Nelson P. G., Goldstone M. W. Electrophysiologic study of cultured neurons dissociated from spinal cords and dorsal root ganglia of fetal mice. Dev Biol. 1973 Jan;30(1):137–152. doi: 10.1016/0012-1606(73)90053-5. [DOI] [PubMed] [Google Scholar]
  21. Rolston J. L. Muscle transplantation into mice with muscular dystrophy. Arch Neurol. 1972 Mar;26(3):258–264. doi: 10.1001/archneur.1972.00490090084007. [DOI] [PubMed] [Google Scholar]
  22. Salafsky B. Functional studies of reeenerated muscles from normal and dystrophic mice. Nature. 1971 Jan 22;229(5282):270–272. doi: 10.1038/229270a0. [DOI] [PubMed] [Google Scholar]
  23. Samaha F. J., Guth L., Albers R. W. The neural regulation of gene expression in the muscle cell. Exp Neurol. 1970 May;27(2):276–282. doi: 10.1016/0014-4886(70)90220-7. [DOI] [PubMed] [Google Scholar]
  24. Shimada Y., Fischman D. A., Moscona A. A. Formation of neuromuscular junctions in embryonic cell cultures. Proc Natl Acad Sci U S A. 1969 Mar;62(3):715–721. doi: 10.1073/pnas.62.3.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sica R. E., McComas A. J. An electrophysiological investigation of limb-girdle and facioscapulohumeral dystrophy. J Neurol Neurosurg Psychiatry. 1971 Aug;34(4):469–474. doi: 10.1136/jnnp.34.4.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Swash M. The morphology and innervation of the muscle spindle in dystrophia myotonica. Brain. 1972;95(2):357–368. doi: 10.1093/brain/95.2.357. [DOI] [PubMed] [Google Scholar]
  27. Wilson B. W., Kaplan M. A., Merhoff W. C., Mori S. S. Innervation and the regulation of acetylcholinesterase activity during the development of normal and dystrophic chick muscle. J Exp Zool. 1970 May;174(1):39–54. doi: 10.1002/jez.1401740105. [DOI] [PubMed] [Google Scholar]
  28. Yaffe D. Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc Natl Acad Sci U S A. 1968 Oct;61(2):477–483. doi: 10.1073/pnas.61.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]

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