Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1981 Oct;34(10):1128–1131. doi: 10.1136/jcp.34.10.1128

Diffusion in gel-enzyme-linked immunosorbent assay—a new serological test for leptospirosis

RTM Cursons 1,2, PA Pyke 1,2
PMCID: PMC494378  PMID: 7309893

Abstract

A new serological test, diffusion in gel-enzyme-linked immunosorbent assay (DIG-ELISA) was developed and compared with the microscopic agglutination test (MAT) for the serological diagnosis of leptospirosis. The results suggest that DIG-ELISA is a viable alternative to the MAT because of its simplicity, sensitivity, versatility and potential for standardisation.

Full text

PDF
1128

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler B., Faine S. Serological and protective-antibody responses of rabbits to leptospiral antigens. J Med Microbiol. 1978 Nov;11(4):401–409. doi: 10.1099/00222615-11-4-401. [DOI] [PubMed] [Google Scholar]
  2. Adler B., Faine S. The antibodies involved in the human immune response to leptospiral infection. J Med Microbiol. 1978 Nov;11(4):387–400. doi: 10.1099/00222615-11-4-387. [DOI] [PubMed] [Google Scholar]
  3. Elwing H., Lange S., Nygren H. Diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA): optimal conditions for quantitation of antibodies. J Immunol Methods. 1980;39(3):247–256. doi: 10.1016/0022-1759(80)90059-9. [DOI] [PubMed] [Google Scholar]
  4. Elwing H., Nygren H. Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA): a simple method for quantitation of class-specific antibodies. J Immunol Methods. 1979;31(1-2):101–107. doi: 10.1016/0022-1759(79)90290-4. [DOI] [PubMed] [Google Scholar]
  5. Turner L. H. Leptospirosis. Br Med J. 1973 Mar 3;1(5852):537–540. doi: 10.1136/bmj.1.5852.537. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES