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. 2016 May 30;5(5):e226. doi: 10.1038/oncsis.2016.32

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Copyright © 2016 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ROR1 and ROR2 silencing inhibits cell migration through transwells. (a) Relative cell migration performed using the transwell migration assay is significantly decreased following ROR1 knockdown in A2780-cis cells. Results represent an average of three experiments. Error bars represent the s.d. of the mean. **P<0.01. (b) Representative images of transwell membranes stained with crystal violet shows decreased number of cells after ROR1 knockdown. (c) Relative cell migration is significantly decreased following ROR2 knockdown in A2780-cis cells. Results represent an average of three experiments. Error bars represent the s.d. of the mean. **P<0.01. (d) Representative images of transwell membranes stained with crystal violet shows decreased number of cells after ROR2 knockdown. (e) Relative cell migration is most significantly decreased following double ROR1 and ROR2 knockdown in A2780-cis cells. Results represent an average of three experiments. Error bars represent the s.d. of the mean. **P<0.01. (f) Representative images of transwell membranes stained with crystal violet shows the strongest decrease in cell number after double ROR1 and ROR2 knockdown.