Figure 3.

The tH is necessary for a Ras protein to promote cell proliferation. 1 × 10⁴ NIH3T3 cells stably expressing the indicated Ras mutants were seeded in triplicate wells in media with low serum. (a) Cells were harvested and counted at the indicated intervals. Cells were harvested and counted 5 h after seeding for 0-h time points. (b) Cells were plated in the presence of either 20 μm LY294002 or 30 μm U0126 as indicated and counted at 72 h. *P<0.01; **P<0.002, n.s., not significant. Representative of five independent experiments.