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. 1992 Jul 15;89(14):6232–6236. doi: 10.1073/pnas.89.14.6232

Targeted oncogene activation by site-specific recombination in transgenic mice.

M Lakso 1, B Sauer 1, B Mosinger Jr 1, E J Lee 1, R W Manning 1, S H Yu 1, K L Mulder 1, H Westphal 1
PMCID: PMC49474  PMID: 1631115

Abstract

An efficient and accurate method for controlled in vivo transgene modulation by site-directed recombination is described. Seven transgenic mouse founder lines were produced carrying the murine lens-specific alpha A-crystallin promoter and the simian virus 40 large tumor-antigen gene sequence, separated by a 1.3-kilobase-pair Stop sequence that contains elements preventing expression of the large tumor-antigen gene and Cre recombinase recognition sites. Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or the human cytomegalovirus promoter. All double-transgenic offspring developed lens tumors. Subsequent analysis confirmed that tumor formation resulted from large tumor-antigen activation via site-specific, Cre-mediated deletion of Stop sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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