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. 1994 Sep;47(9):793–795. doi: 10.1136/jcp.47.9.793

Circulating fibronectin and fibronectin receptor in children with pertussis.

D Torre 1, M Giola 1, C Zeroli 1, R Martegani 1, G Bonetta 1, G Ferrario 1
PMCID: PMC494933  PMID: 7962645

Abstract

AIM--To determine concentrations of fibronectin and fibronectin receptor in children with pertussis. METHODS--Concentrations of circulating fibronectin and serum fibronectin receptor were detected in eight children affected by pertussis, eight children with acute upper or lower respiratory tract infections, and in 14 healthy control children. The single radial immunodiffusion technique and a solid phase enzyme immunoassay were used to detect circulating serum concentrations of fibronectin and fibronectin receptor. RESULTS--On admission, a significant decrease in fibronectin was detected in children with pertussis (p = 0.0006). Significant and decreased concentrations of fibronectin were also observed in children with upper or lower respiratory tract infections (p = 0.0002). On the other hand, serum fibronectin receptor concentrations were significantly increased in patients with pertussis, whereas patients with upper or lower respiratory tract infections had normal circulating fibronectin receptor concentrations. CONCLUSIONS--Fibronectin deficiency in children with pertussis may be related to diffusion and deposition of this protein in bronchial and alveolar spaces to limit infection, while increased fibronectin receptor concentrations are probably the expression of T cell activation and cell-mediated immunity during Bordetella pertussis infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beachey E. H., Courtney H. S. Bacterial adherence: the attachment of group A streptococci to mucosal surfaces. Rev Infect Dis. 1987 Sep-Oct;9 (Suppl 5):S475–S481. doi: 10.1093/clinids/9.supplement_5.s475. [DOI] [PubMed] [Google Scholar]
  2. Cardarelli P. M., Blumenstock F. A., Saba T. M., Rourke F. J. Fibronectin-enhanced attachment of gelatin-coated erythrocytes to isolated hepatic Kupffer cells. J Leukoc Biol. 1984 Oct;36(4):477–492. doi: 10.1002/jlb.36.4.477. [DOI] [PubMed] [Google Scholar]
  3. Charles I. G., Dougan G., Pickard D., Chatfield S., Smith M., Novotny P., Morrissey P., Fairweather N. F. Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis. Proc Natl Acad Sci U S A. 1989 May;86(10):3554–3558. doi: 10.1073/pnas.86.10.3554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cheers C., Gray D. F. Macrophage behaviour during the complaisant phase of murine pertussis. Immunology. 1969 Dec;17(6):875–887. [PMC free article] [PubMed] [Google Scholar]
  5. Friedman R. L., Nordensson K., Wilson L., Akporiaye E. T., Yocum D. E. Uptake and intracellular survival of Bordetella pertussis in human macrophages. Infect Immun. 1992 Nov;60(11):4578–4585. doi: 10.1128/iai.60.11.4578-4585.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gray D. F., Cheers C. The steady state in cellular immunity. II. Immunological complaisance in murine pertussis. Aust J Exp Biol Med Sci. 1967 Aug;45(4):417–426. doi: 10.1038/icb.1967.40. [DOI] [PubMed] [Google Scholar]
  7. Hynes R. O. Integrins: a family of cell surface receptors. Cell. 1987 Feb 27;48(4):549–554. doi: 10.1016/0092-8674(87)90233-9. [DOI] [PubMed] [Google Scholar]
  8. Hynes R. Molecular biology of fibronectin. Annu Rev Cell Biol. 1985;1:67–90. doi: 10.1146/annurev.cb.01.110185.000435. [DOI] [PubMed] [Google Scholar]
  9. Kuusela P. Fibronectin binds to Staphylococcus aureus. Nature. 1978 Dec 14;276(5689):718–720. doi: 10.1038/276718a0. [DOI] [PubMed] [Google Scholar]
  10. Limper A. H., Roman J. Fibronectin. A versatile matrix protein with roles in thoracic development, repair and infection. Chest. 1992 Jun;101(6):1663–1673. doi: 10.1378/chest.101.6.1663. [DOI] [PubMed] [Google Scholar]
  11. Mills K. H., Barnard A., Watkins J., Redhead K. Cell-mediated immunity to Bordetella pertussis: role of Th1 cells in bacterial clearance in a murine respiratory infection model. Infect Immun. 1993 Feb;61(2):399–410. doi: 10.1128/iai.61.2.399-410.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mosher D. F. Physiology of fibronectin. Annu Rev Med. 1984;35:561–575. doi: 10.1146/annurev.me.35.020184.003021. [DOI] [PubMed] [Google Scholar]
  13. Petersen J. W., Ibsen P. H., Hasløv K., Heron I. Proliferative responses and gamma interferon and tumor necrosis factor production by lymphocytes isolated from tracheobroncheal lymph nodes and spleen of mice aerosol infected with Bordetella pertussis. Infect Immun. 1992 Nov;60(11):4563–4570. doi: 10.1128/iai.60.11.4563-4570.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts C. J., Birkenmeier T. M., McQuillan J. J., Akiyama S. K., Yamada S. S., Chen W. T., Yamada K. M., McDonald J. A. Transforming growth factor beta stimulates the expression of fibronectin and of both subunits of the human fibronectin receptor by cultured human lung fibroblasts. J Biol Chem. 1988 Apr 5;263(10):4586–4592. [PubMed] [Google Scholar]
  15. Ruoslahti E., Pierschbacher M. D. New perspectives in cell adhesion: RGD and integrins. Science. 1987 Oct 23;238(4826):491–497. doi: 10.1126/science.2821619. [DOI] [PubMed] [Google Scholar]
  16. Scheld W. M., Strunk R. W., Balian G., Calderone R. A. Microbial adhesion to fibronectin in vitro correlates with production of endocarditis in rabbits. Proc Soc Exp Biol Med. 1985 Dec;180(3):474–482. doi: 10.3181/00379727-180-42205. [DOI] [PubMed] [Google Scholar]
  17. Tamkun J. W., Hynes R. O. Plasma fibronectin is synthesized and secreted by hepatocytes. J Biol Chem. 1983 Apr 10;258(7):4641–4647. [PubMed] [Google Scholar]
  18. Torikata C., Villiger B., Kuhn C., 3rd, McDonald J. A. Ultrastructural distribution of fibronectin in normal and fibrotic human lung. Lab Invest. 1985 Apr;52(4):399–408. [PubMed] [Google Scholar]
  19. Tuomanen E., Towbin H., Rosenfelder G., Braun D., Larson G., Hansson G. C., Hill R. Receptor analogs and monoclonal antibodies that inhibit adherence of Bordetella pertussis to human ciliated respiratory epithelial cells. J Exp Med. 1988 Jul 1;168(1):267–277. doi: 10.1084/jem.168.1.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vercellotti G. M., Lussenhop D., Peterson P. K., Furcht L. T., McCarthy J. B., Jacob H. S., Moldow C. F. Bacterial adherence to fibronectin and endothelial cells: a possible mechanism for bacterial tissue tropism. J Lab Clin Med. 1984 Jan;103(1):34–43. [PubMed] [Google Scholar]
  21. Wiertz E. J., Walvoort H. C., Van Loveren H., Van Straaten-Van De Kappelle I., Van Der Gun J. W., Kreeftenberg J. G. Acellular and whole cell pertussis vaccines protect against the lethal effects of intracerebral challenge by two different T-cell dependent humoral routes. Biologicals. 1990 Jul;18(3):173–180. doi: 10.1016/1045-1056(90)90004-j. [DOI] [PubMed] [Google Scholar]

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