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. 1994 Sep;47(9):862–864. doi: 10.1136/jcp.47.9.862

In situ hybridisation for the identification of Helicobacter pylori in paraffin wax embedded tissue.

M S Bashir 1, F A Lewis 1, P Quirke 1, A Lee 1, M F Dixon 1
PMCID: PMC494950  PMID: 7525660

Abstract

A method for identifying Helicobacter pylori using a non-isotopic in situ hybridisation technique is described. A probe generated by polymerase chain reaction (PCR) with primers directed against parts of the Helicobacter pylori 16SrRNA sequence was used. Paraffin wax embedded gastric biopsy specimens from patients with and without gastritis were hybridised with the probe, and the method was shown to be sensitive and specific for H pylori.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Engstrand L., Pählson C., Gustavsson S., Schwan A. Monoclonal antibodies for rapid identification of Campylobacter pyloridis. Lancet. 1986 Dec 13;2(8520):1402–1403. doi: 10.1016/s0140-6736(86)92049-0. [DOI] [PubMed] [Google Scholar]
  2. Goodwin C. S., Blincow E. D., Warren J. R., Waters T. E., Sanderson C. R., Easton L. Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol. 1985 Oct;38(10):1127–1131. doi: 10.1136/jcp.38.10.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ho S. A., Hoyle J. A., Lewis F. A., Secker A. D., Cross D., Mapstone N. P., Dixon M. F., Wyatt J. I., Tompkins D. S., Taylor G. R. Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals. J Clin Microbiol. 1991 Nov;29(11):2543–2549. doi: 10.1128/jcm.29.11.2543-2549.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lewis F. A., Griffiths S., Dunnicliff R., Wells M., Dudding N., Bird C. C. Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex. J Clin Pathol. 1987 Feb;40(2):163–166. doi: 10.1136/jcp.40.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. McNulty C. A., Dent J. C., Uff J. S., Gear M. W., Wilkinson S. P. Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients. Gut. 1989 Aug;30(8):1058–1062. doi: 10.1136/gut.30.8.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morotomi M., Hoshina S., Green P., Neu H. C., LoGerfo P., Watanabe I., Mutai M., Weinstein I. B. Oligonucleotide probe for detection and identification of Campylobacter pylori. J Clin Microbiol. 1989 Dec;27(12):2652–2655. doi: 10.1128/jcm.27.12.2652-2655.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Van den Berg F. M., Zijlmans H., Langenberg W., Rauws E., Schipper M. Detection of Campylobacter pylori in stomach tissue by DNA in situ hybridisation. J Clin Pathol. 1989 Sep;42(9):995–1000. doi: 10.1136/jcp.42.9.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wetherall B. L., McDonald P. J., Johnson A. M. Detection of Campylobacter pylori DNA by hybridisation with non-radioactive probes in comparison with a 32P-labelled probe. J Med Microbiol. 1988 Aug;26(4):257–263. doi: 10.1099/00222615-26-4-257. [DOI] [PubMed] [Google Scholar]

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