Table 2.
Yeast strains used in this study
| Name | Genotype | Background | Source |
|---|---|---|---|
| BY4741 | his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; Mat a | BY474x | EUROSCARF |
| BY4743 | his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 lys2D0/LYS2; diploid | BY474x | EUROSCARF |
| MKY2128 | his3Δ1 leu2Δ0 ura3Δ0 can1::Ste2pr‐Leu2 lyp1Δ tor1‐1 fpr1::klURA; Matα | Y7039 | Gallego et al., 2013 |
| yAB2 | ATG17‐protAH3HA:URA | BY474x | This study |
| yAB5 | ATG13‐protAH3HA:URA | BY474x | This study |
| yAB7 | ATG17‐protAH3HA:URA atg13::KANMX6 | BY474x | This study |
| yAB66 | ATG1‐CNB1:NATMX6 fpr1::URA cnb1::KANMX6 ATG13‐protAH3HA:URA | BY474x | This study |
| yAB67 | ATG17‐H3HA:URA atg13::KANMX6, MET15, Matα | BY474x | This study |
| yAB68 | ATG17‐GFP:HISMX6 | BY474x | LifeTechnologies |
| yLW25 | ATG1‐H3HA:URA atg13::KANMX6 | BY474x | This study |
| yLW43 | ATG1‐protAH3HA:URA | BY474x | This study |
| yTB12 | cnb1::KANMX6/CNB1; diploid | BY474x | EUROSCARF |
| yTM26 | ATG1‐CNB1 cnb1::KANMX6 fpr1::URA, Matα | BY474x | This study |
All strains of S.cerevisiae S288C BY474x genetic background are derived from the diploid strain BY4743 and carry the following markers: his3Δ1, leu2Δ0, met15Δ0, ura3Δ0, Mat a, unless stated otherwise.