Skip to main content
Journal of Advanced Research logoLink to Journal of Advanced Research
. 2016 Jul 2;7(5):635–641. doi: 10.1016/j.jare.2016.06.009

Dynamic light scattering of nano-gels of xanthan gum biopolymer in colloidal dispersion

Abbas Rahdar a,b,, Mohammad Almasi-Kashi a,c
PMCID: PMC4950164  PMID: 27489730

Graphical abstract

graphic file with name fx2.jpg

Keywords: Xanthan gum, Interaction, Dynamic light scattering, Nano-droplets, Mass fraction of droplet, Microemulsion

Abstract

The dynamical properties of nanogels of xanthan gum (XG) with hydrodynamic radius controlled in a size range from 5 nm to 35 nm, were studied at the different XG concentrations in water/sodium bis-2-ethylhexyl-sulfosuccinate (AOT)/decane reverse micelles (RMs) vs. mass fraction of nano-droplet (MFD) at W = 40, using dynamic light scattering (DLS). The diffusion study of nanometer-sized droplets by DLS technique indicated that enhancing concentration of the XG polysaccharide resulted in exchanging the attractive interaction between nano-gels to repulsive interaction, as the mass fraction of nano-droplets increased. The reorientation time (τr) of water nanodroplets decreased with MFD for water-in-oil AOT micro-emulsion comprising high concentration (0.0000625) of XG. On the other hand, decreasing concentration of biopolymer led to increasing the rotational correlation time of water nanodroplets with MFD. In conclusion, a single relaxation curve was observed for AOT inverse microemulsions containing different XG concentrations. Furthermore, the interaction between nanogels was changed from attractive to repulsive versus concentration of XG in the AOT RMs.

Introduction

Due to their tunable chemical properties, biocompatibility, and flexible three-dimensional physical structures, hydrogels or aquagels, networks of water-soluble polymers, are applied in various fields of study, including pharmaceutical engineering, biomaterials science, and biomedical engineering [1], [2], [3], [4]. Nano-hydrocolloids in submicron dimensions are developed to obtain excellent advantages for drug delivery purposes via conjugating the polymeric networks with additives, such as drugs and proteins [5], [6]. The synthesis of nano-sized hydrogels has attracted substantial attention in the recent years and different methods, such as biopolymers modification [7], [8], [9], [10], [11], free radical polymerization [12], microfluidics [13], and polymerization via reverse microemulsion [14], [15], [16], [17] have been employed in the preparation of nanogels. The aforementioned methods have some deficiencies and superiorities. For instance, it was discovered through an eloquent series of studies that the exact controlling of the size of the hydrogels generated via the polymerization method in the inverse microemulsions avoids the variation in the hydrogel morphology and multi-steps synthesis present in other reported methods [7], [8], [9], [10], [11], [12], [13]. Moreover, different research groups have recently focused on the reverse micelles approach due to their wide application as a matrix to synthesize the nano-sized hydrogel particles and nano reactor for aqueous reactants [14], [15], [16], [17].

These generated nanogels are networks of hydrophilic polymers, which are easily assembled in the aqueous core of reverse micelles. It is noteworthy that the size and shape of the nanogels obtained from the polymerization approach via the droplet reverse microemulsions are easily managed by the polar solvent-to-surfactant molar ratio [14], [15], [16], [17]. In some practical applications, additives (i.e. drugs, DNA, magnetic particles, and cells) can be physically bonded inside the water nanodroplets, and these generated nanodroplets are stabilized via inclusion of a surface-active agent, such as AOT in bulk non-polar solvent to afford the final in reverse microemulsion [14], [15], [16], [17]. Accordingly, the above-mentioned background, in the XG-loaded AOT reverse micelles (RMs), nano-sized water droplets containing XG (nanogels) are formed and dispersed by surfactant film of sodium di-2-ethylhexylsulfosuccinate (AOT, Aerosol OT) in the bulk apolar solvent of decane oil. Herein, the dynamic behavior of the AOT RM system contains polymer influenced by the interaction present between the polymer and the droplets or surfactant that in turn leads to adsorbing or non-adsorbing polymers in the water/surfactant interface or the water nano-droplet core, respectively [18], [19], [20], [21]. Moreover, the length scale and molecular weight effects of different polymers on AOT RM system have been thoroughly investigated [18], [19], [20], [21], [22], [23]. However, the underlying details on preparation of nanogels of XG biopolymer at various concentrations in the water-in-decane AOT reverse micelle systems were not notified, to the best of the author’s knowledge.

It is important to mention that XG is a hydrophilic polysaccharide with cellulose-like backbone fabricated by the Xanthomonas campestris. The preliminary structure of this biopolymer contains side chains and cellulose-like chain (Fig. 1) [24]. The anionic portion of XG biopolymer is due to the presence of the pyruvic and glucuronic groups in the side chains [25]. The XG is a biopolymer with a widespread range of usages in various fields, such as pharmaceutical, food, agricultural, and textile industries owing to its rheological properties. For instance, the XG has received a growing attention in various fields such as pharmaceutical formularization as a disintegrant, gelling agent, and binder because of its high viscosity at the polymer low concentrations. In addition, this polymeric species has been utilized as the key agent in the controlling and retarding process of drug release due to its gelling character and ability of encapsulating the drug within the gel as well as the drug delivery to the target area without creating a toxic effect [26]. Furthermore, the natural polysaccharides, such as xanthan and guar gums are selectively degraded in the colon, but not in the stomach and/or in small intestine [27]. Thus, utilizing these biopolymers in colon drug delivery is a good approach in treatment of colon-associated diseases [28]. Accordingly, in the present work, the dynamic of the nano-scale water droplets comprising XG in the water/AOT/decane RMs system at different XG concentrations and at W = [H2O/AOT] = 40 as a function of MFD using the DLS technique was investigated.

Fig. 1.

Fig. 1

Chemical structure of xanthan gum.

Experimental

Materials and preparation of entrapment–XG water nanodroplet (XG nanogels)

The AOT (purity > 99%), decane (purity > 95%), and XG were purchased from Sigma–Aldrich (Taufkirchen, Germany).

To prepare the AOT-templated nanogels of XG, the weighed powder of the biopolymer was initially dissolved in deionized water with a certain concentration (namely, 0.001) at room temperature. The polymer-to-water mass ratio, Y = mpolymer/mH2O [29], was specified as the concentration of XG in the AOT RMs. The water-containing AOT inverse micelles were prepared via mixing the appropriate mass value of the AOT, decane, and water containing different concentrations of XG at the fixed H2O-to-AOT molar ratio (W = 40). In the final step, the AOT RM was diluted with non-polar solvent of decane following the certain mass fraction of nano-droplet [29] at room temperature (RT).

Theory of dynamic light scattering

To study the inter-nanodroplet interactions by the collective diffusion coefficient of AOT nano-micelles, the DLS technique (also known as Quasi-Elastic Light Scattering (QELS)) or Photon Correlation Spectroscopy (PCS), was used to characterize the size distribution of nanometer-sized water droplets by a digital correlator [30], [31], [32], [33], [34]. The DLS technique utilized the time auto-correlation function to analyze the modulation of the scattered light intensity passing through a colloidal solution comprising submicron droplets. The scattered light intensity of AOT RMs was then monitored with time, which depends on the droplet size, Brownian motion of nano-droplets and their diffusive behavior in solution as well as viscosity of the continuous phase. Dynamic light scattering of the dispersed droplets was then investigated based on the fluctuation of the scattered intensity of I(t). The autocorrelation function of the scattered light, g2(q, τ), of AOT RMs was studied between the intensity of I(t) at t and I [30], [31], [32], [33], [34] using the following Eq. (1).

g2(τ)=I(t)I(t+τ)I(t)2 (1)

According to Eq. (1), the correlation between the signal intensities at t and t + τ increased as τ decreased and consequently g2(τ) tends toward 1 [30], [31], [32], [33], [34]. The required time for decaying the auto-correlation function of micelles to zero was dependent on the size distribution of nano-droplets. Notably, the normalized auto-correlation function of g2(τ) was related to the auto-correlation function of the scattered light electric field, g1(τ), following the Siegert relationship [30], [31], [32], [33], [34].

g2(τ)-1=A|g1(0)exp(-DQ2)|2 (2)

In which, A (0 < A < 1) was the experimental coherence factor, D was the diffusion coefficient and Q was the scattering vector. The magnitude of the scattering vector was related (Eq. (3)) to the wavelength of the X-ray or laser, the scattering angle of θ, and n, the refractive index of solvent [30], [31], [32], [33], [34].

Q=4πnλsinθ2 (3)

Notably, when the particles were in mono-dispersed form, the autocorrelation function of g1(τ), exhibited a single exponential decay curve [30], [31], [32], [33], [34].

g1(τ)=g1(0)exp(-DQ2) (4)

where DQ2 defined as the inverse correlation time namely Γ [30], [31], [32], [33], [34], [35], [36], [37], [38], [39], [40], [41], [42], [43], [44].

D=Γ/Q2 (5)

It is important to note that as the fluctuations in light intensity changed more slowly, increasing the sizes of droplets resulted in a slower relaxing exponential with a smaller relax constant, whereas decreasing the sizes of droplet led to a rapidly relaxing exponential function with a large relax constant. Therefore, the inverse correlation time is inversely proportional to the nano-droplet size [29], [30], [31], [32], [33], [34], [35], [36], [37], [38], [39], [40].

Finally, the diffusion coefficient of AOT micelles was changed to the hydrodynamic radius following the Stokes-Einstein relation [30], [31], [32], [33], [34]:

rh=KBT6ηπD (6)

in which, T was the temperature in K, KB was a Boltzmann’s constant, and η was the viscosity of the continuous phase in the AOT RMs.

The reorientation time, τr, for spherical droplets containing xanthan gum in the AOT reverse micelles was obtained based on the Stokes-Einstein-Debye (SED) relation [30], [31], [32], [33], [34].

τr=4πηrh33KBT (7)

where KB was Boltzmann’s constant, rh was the hydrodynamic radius of nano-scale droplets, T was the temperature in K, and η was the viscosity of the bulk phase in the water/AOT/decane microemulsion.

Results and discussion

The correlation function with relaxation time for W/O droplet microemulsions containing XG is depicted in Fig. 2, Fig. 3, Fig. 4. As observed from Fig. 2, the auto-correlation function of micelle at MFD = 0.1, showed a shorter diffusion time compared with the other AOT reverse micelles. A through comparison of Fig. 2, Fig. 3, Fig. 4 revealed that the auto-correlation function of AOT water-in-oil microemulsion droplet at MFD = 0.1, exhibited shorter diffusion time compared with the other AOT reverse micelles. To obtain the relaxation rate, Γ, and the diffusion coefficient, D, of nano-scale water droplets, the auto-correlation function was fitted with a single exponential curve following Eq. (2). The relaxation rate of AOT inverse microemulsion versus MFD is shown in Fig. 1s. According to this figure, various concentrations of XG in the AOT water nanodroplets versus MFD resulted in changing the inverse correlation time of AOT RMs.

Fig. 2.

Fig. 2

The autocorrelation function versus time for AOT reversed nano-micelles at polymer concentration of 0.0000625 at RT.

Fig. 3.

Fig. 3

The autocorrelation function versus time for AOT reversed nano-micelles at polymer concentration of 0.0000157 at RT.

Fig. 4.

Fig. 4

The autocorrelation function versus time for AOT reverse nano-micelles at polymer concentration of 0.0000079 at RT.

In general, it was found that by changing XG concentration in the AOT reverse micelles, variation of the size and diffusion of water droplets as well as the inter-nanogel interactions, versus mass fraction of droplet was observed. A careful analysis of diffusion of the AOT RMs comprising XG versus MFD, revealed that the collective diffusion for AOT micelles containing XG showed a negative slope as a function of MFD at concentration value of 0.0000079 and a positive slope at concentration of 0.0000625 (Fig. 5). Importantly, the nature of interaction of AOT droplets changed from attractive to repulsive force as concentration of XG biopolymer increased as a function of MFD. This observation was due to the fact that adsorbing XG polysaccharide at interfacial of AOT micelles, induced repulsive interaction of the droplet-droplet as a consequence of the increasing concentration of XG biopolymer in the AOT RMs [35], [45]. On the other hand, non-adsorbing the XG biopolymer in core of droplets and decreasing the concentration of XG polysaccharide in the AOT RMs induced an attractive interaction between water droplets based on Asakura–Oosawa (AO) model of depletion interaction [35]. The fact that changing the size of droplet and the inter-droplet interactions affected changing content of oil in the RMs system has been proved by other research groups [29], [36], thus further supporting the current observations.

Fig. 5.

Fig. 5

Diffusion coefficient of AOT RMs containing different xanthan gum concentrations as a function of MFD (up triangle): 0.0000625, (circle): 0.0000157 and (down triangle): 0.0000079 at RT.

It is worth to note that in the spherical water-comprising AOT reverse micelles, H2O/AOT interactions were less desirable than H2O/H2O and/or AOT/AOT interactions due to size effects in the nanometer-sized structures [42], [43]. It is well known that both sizes and interactions of water-soluble macromolecules affected the dynamics of water droplets in Reverse micelles [44], [45]. As observed in Fig. 6, the average size of nanogels was determined by interpreting the diffusion coefficient of micelles, as the hydrodynamic radius using the Stokes-Einstein relation. As is apparent in Fig. 6, the hydrodynamic size of water droplets decreased as polysaccharide concentration of XG increased in the AOT inverse microemulsion. Notably, change of size of the micellar core via adding an additive in the water-comprising AOT reversed micelles has been reported by others [29], [41].

Fig. 6.

Fig. 6

Hydrodynamic diameter of AOT water nanodroplets containing different xanthan gum concentrations using Stokes-Einstein Relation versus MFD (up triangle): 0.0000625, (circle): 0.0000157 and (down triangle): 0.0000079, at RT.

To support this phenomena, it was proposed that (i) biopolymer of XG acted as co-surfactants; thus, by increasing the concentration of the XG biopolymer in the AOT RMs, the interfacial surface of AOT micelles increased. This led to enhancing the number of water nanodroplets and decreasing their sizes, as observed experimentally. (ii) Some parts of XG or their ends may be adsorbed to the surface of the AOT molecules as an active agent at the interfacial of AOT micelles, while the other parts may be non-adsorbed in the core of water droplets. After this observation, the elastic energy of XG molecules may result in decreasing the size of the droplets [37], [38]. (iii) Furthermore, reduction in droplet sizes was attributed to decrease in overlapping the interface domain of inter-droplets, which in turn causes the decreased strength of the attractive interaction [37], [38]. If the elastic modulus as only factor in change of the droplets size to be considered, therefore, the nano-droplet sizes decreased as the interface curvature increased due to the surface-to-volume effects in nano-scale systems [39].

The size distribution of AOT nanometer-sized water droplet comprising the different xanthan gum concentration obtained from DLS measurement with MFD is shown in Figs. 2–4s.

The reorientation time (τr) of water nano-droplets containing the xanthan gum biopolymer versus MFD achieved by the Stokes-Einstein-Debye (SED) relation is also shown in Fig. 7. As observed, changing the reorientation time of micelles upon an external perturbation such as inclusion of an additive resulted in changing in fluidity of a system well [40]. Moreover, according to this figure, for AOT water-in-oil droplet microemulsion containing XG with high concentration (0.0000625), the reorientation time (τr) of water nanodroplets decreased with MFD, whereas decreasing the concentration of biopolymer, the rotational correlation time of water nanodroplets increased versus mass fraction of water nanodroplet.

Fig. 7.

Fig. 7

Rotational correlation time (τr) versus MFD for water nano-droplets containing different concentrations of XG in AOT RM at concentration (circle): 0.0000625, (star):0.0000157 and (down triangle):0.0000079 using the SED relation.

Conclusions

To sum up, the diffusion of nano-scale hydrogels comprising the different XG concentrations at the H2O-to-AOT molar ratio of 40 (W = 40) and different mass fraction of nano-droplets was studied by dynamic light scattering technique in water-in-decane droplet microemulsion. The nano-gels were formed and dispersed in the bulk phase of decane based on the anionic surfactant of AOT (sodium bis[2-ethylhexyl] sulfosuccinate). A single relaxation curve was observed for nanogels, further supporting the fact that addition of XG to the water-comprising AOT reversed micelles. As highlighted by the analysis of dynamic light scattering of micelles, with increasing the concentration of the XG biopolymer, the diffusion coefficient of nanogels increased and size of droplets decreased as mass fraction of droplets increased. Lastly, it was found that enhancing the concentration of XG in the AOT micelles changed the nature of inter-nanogels interaction from an attractive to repulsive force.

Conflict of Interest

The authors have declared no conflict of interest.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

Acknowledgments

The authors would like to thank University of Kashan for financial support for this work.

Footnotes

Peer review under responsibility of Cairo University.

Appendix A

Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.jare.2016.06.009.

Appendix A. Supplementary material

Supplementary figures
mmc1.doc (1.7MB, doc)

References

  • 1.Ahmed E.M. Hydrogel: preparation, characterization, and applications: a review. J Adv Res. 2015;6(2):105–121. doi: 10.1016/j.jare.2013.07.006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Deligkaris K., Tadele T.S., Olthuis W., van den Berg A. Hydrogel-based devices for biomedical applications. Sensor Actuat B – Chem. 2010;147(2):765–774. [Google Scholar]
  • 3.Hoffman A.S. Hydrogels for biomedical applications. Adv Drug Deliv Rev. 2002;54(1):3–12. doi: 10.1016/s0169-409x(01)00239-3. [DOI] [PubMed] [Google Scholar]
  • 4.Hoare T.R., Kohane D.S. Hydrogels in drug delivery: progress and challenges. Polymer. 2008;49(8):1993–2007. [Google Scholar]
  • 5.Harris J.M., Chess R.B. Effect of pegylation on pharmaceuticals. Nat Rev Drug Discov. 2003;2(3):214–221. doi: 10.1038/nrd1033. [DOI] [PubMed] [Google Scholar]
  • 6.Vicent M.J., Greco F., Nicholson R.I., Paul A., Griffiths P.C., Duncan R. Polymer therapeutics designed for a combination therapy of hormone-dependent cancer. Angew Chem Int Ed Engl. 2005;44(26):4061–4066. doi: 10.1002/anie.200462960. [DOI] [PubMed] [Google Scholar]
  • 7.Nishikawa T., Akiyoshi K., Sunamoto J. Macromolecular complexation between bovine serum albumin and the self-assembled hydrogel nanoparticle of hydrophobized polysaccharides. J Am Chem Soc. 1996;118(26):6110–6115. [Google Scholar]
  • 8.Nishikawa T., Akiyoshi K., Sunamoto J. Supramolecular assembly between nanoparticles of hydrophobized polysaccharide and soluble protein complexation between the self-aggregate of cholesterol-bearing pullulan and alpha-chymotrypsin. Macromolecules. 1994;27(26):7654–7659. [Google Scholar]
  • 9.Lee I., Akiyoshi K. Single molecular mechanics of a cholesterol-bearing pullulan nanogel at the hydrophobic interfaces. Biomaterials. 2004;25(15):2911–2918. doi: 10.1016/j.biomaterials.2003.09.065. [DOI] [PubMed] [Google Scholar]
  • 10.Akiyoshi K., Kang E.C., Kurumada S., Sunamoto J., Principi T., Winnik F.M. Controlled Association of amphiphilic polymers in water: thermosensitive nanoparticles formed by self-assembly of hydrophobically modified pullulans and Poly(N-isopropylacrylamides) Macromolecules. 2000;33(9):3244–3249. [Google Scholar]
  • 11.Bodnar M., Hartmann J.F., Borbely J. Synthesis and study of cross-linked chitosan-N-poly(ethylene glycol) nanoparticles. Biomacromolecules. 2006;7(11):3030–3036. doi: 10.1021/bm0605053. [DOI] [PubMed] [Google Scholar]
  • 12.Ma Z., Lacroix-Desmazes P. Dispersion polymerization of 2-hydroxyethyl methacrylate stabilized by a hydrophilic/CO 2-philic poly(ethylene oxide)-b-poly(1, 1, 2, 2-tetrahydroperfluorodecyl acrylate)(PEO-b-PFDA) diblock copolymer in supercritical carbon dioxide. Polymer. 2004;45(20):6789–6797. [Google Scholar]
  • 13.Zhang H., Tumarkin E., Sullan R.M.A., Walker G.C., Kumacheva E. Exploring microfluidic routes to microgels of biological polymers. Macromol Rapid Commun. 2007;28(5):527–538. [Google Scholar]
  • 14.Oh J.K., Siegwart D.J., Lee H.I., Sherwood G., Peteanu L., Hollinger J.O. Biodegradable nanogels prepared by atom transfer radical polymerization as potential drug delivery carriers: synthesis, biodegradation, in vitro release, and bioconjugation. J Am Chem Soc. 2007;129(18):5939–5945. doi: 10.1021/ja069150l. [DOI] [PubMed] [Google Scholar]
  • 15.Mitra S., Gaur U., Ghosh P.C., Maitra A.N. Tumour targeted delivery of encapsulated dextran-doxorubicin conjugate using chitosan nanoparticles as carrier. J Control Release. 2001;74(1–3):317–323. doi: 10.1016/s0168-3659(01)00342-x. [DOI] [PubMed] [Google Scholar]
  • 16.Lee H., Mok H., Lee S., Oh Y.K., Park T.G. Target-specific intracellular delivery of siRNA using degradable hyaluronic acid nanogels. J Control Release. 2007;119(2):245–252. doi: 10.1016/j.jconrel.2007.02.011. [DOI] [PubMed] [Google Scholar]
  • 17.McAllister K., Sazani P., Adam M., Cho M.J., Rubinstein M., Samulski R.J. Polymeric nanogels produced via inverse microemulsion polymerization as potential gene and antisense delivery agents. J Am Chem Soc. 2002;124(51):15198–15207. doi: 10.1021/ja027759q. [DOI] [PubMed] [Google Scholar]
  • 18.Meier W. Poly(oxyethylene) adsorption in water/oil microemulsions: a conductivity study. Langmuir. 1996;12(5):1188–1192. [Google Scholar]
  • 19.Laia C.A., Brown W., Almgren M., Costa S.M. Light scattering study of water-in-oil AOT microemulsions with poly(oxy)ethylene. Langmuir. 2000;16(2):465–470. [Google Scholar]
  • 20.Maugey M., Bellocq A.M. Effect of added salt and poly(ethylene glycol) on the phase behavior of a balanced AOT−water−oil system. Langmuir. 1999;15(25):8602–8608. [Google Scholar]
  • 21.Papoutsi D., Lianos P., Brown W. Interaction of polyethylene glycol with water-in-oil microemulsions. 3. Effect of polymer size and polymer concentration. Langmuir. 1994;10(10):3402–3405. [Google Scholar]
  • 22.Milas M., Rinaudo M. Properties of xanthan gum in aqueous solutions: role of the conformational transition. Carbohyd Res. 1986;158:191–204. [Google Scholar]
  • 23.Li H., Chen R., Lu X., Hou W. Rheological properties of aqueous solution containing xanthan gum and cationic cellulose JR400. Carbohyd Polym. 2012;90(3):1330–1336. doi: 10.1016/j.carbpol.2012.07.001. [DOI] [PubMed] [Google Scholar]
  • 24.Katzbauer B. Properties and applications of xanthan gum polymer degradation and stability. Polym Degrad Stab. 1998;59(1):81–84. [Google Scholar]
  • 25.Kwon G.S., Moon S.H., Hong S.D., Lee H.M., Mheen T.I., Oh H.M. Rheological properties of extracellular polysaccharide, Pestan, produced by Pestalotiopsis sp. Biotechnol Lett. 1996;18(12):1465–1470. [Google Scholar]
  • 26.Benny I.S., Gunasekar V., Ponnusami V. Review on application of xanthan gum in drug delivery. Int J PharmTech Res. 2014;6(4):1322–1326. [Google Scholar]
  • 27.Salyers A.A., Vercellotti J.R., West S.E., Wilkins T.D. Fermentation of mucin and plant polysaccharides by strains of bacteroides from the human colon. Appl Environ Microbiol. 1977;32(2):319–322. doi: 10.1128/aem.33.2.319-322.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Ramasamy T., Kandhasami U.D., Ruttala H., Shanmugam S. Formulation and evaluation of xanthan gum based aceclofenac tablets for colon targeted drug delivery. Braz J Pharm Sci. 2011;47(2):299–311. [Google Scholar]
  • 29.Rahdar A., Almasi-Kashi M. Photophysics of Rhodamine B in the nanosized water droplets: a concentration dependence study. J Mol Liq. 2016;220:395–403. [Google Scholar]
  • 30.Brown W. Clarendon Press; Oxford: 1993. Dynamic light scattering: the method and some applications. [Google Scholar]
  • 31.Finsy R. Particle sizing by quasi-elastic light scattering. Adv Colloid Interface Sci. 1994;52:79–143. [Google Scholar]
  • 32.Pecora P. Plenum Press; New York: 1985. Dynamic light scattering applications of photon correlation spectroscopy. [Google Scholar]
  • 33.Zhu R., Lu R., Yu A. Photophysics and locations of IR125 and C152 in AOT reverse micelles. Phys Chem Chem Phys. 2011;13(64):20844–20854. doi: 10.1039/c1cp21946d. [DOI] [PubMed] [Google Scholar]
  • 34.Valeur B., Berberan-Santos M.N. 2nd ed. Wiley-VCH; Germany: 2012. Molecular fluorescence: principles and applications. [Google Scholar]
  • 35.Cosgrove T. 2nd ed. John Wiley and Sons; United Kingdom: 2010. Colloid Science: principles, methods and applications. [Google Scholar]
  • 36.Florez Tabares J.S., Correa N.M., Silber J.J., Sereno L.E., Molina P.G. Droplet–droplet interactions investigated using a combination of electrochemical and dynamic light scattering techniques. The case of water/BHDC/benzene: n-heptane system. Soft Matter. 2015;11(15):2952–2962. doi: 10.1039/c5sm00146c. [DOI] [PubMed] [Google Scholar]
  • 37.Suarez M.J., Lang J. Effect of Addition of water-soluble polymers in water-in-oil microemulsions made with anionic and cationic surfactants. J Phys Chem. 1995;99(13):4626–4631. [Google Scholar]
  • 38.Suarez M.J., Levy H., Lang J. Effect of addition of polymer to water-in-oil microemulsions on droplet size and exchange of material between droplets. J Phys Chem. 1993;97(38):9808–9816. [Google Scholar]
  • 39.Stubenrauch C. 1st ed. John Wiley and Sons; United Kingdom: 2009. Microemulsions: background, new concepts, applications, perspectives. [Google Scholar]
  • 40.Eastoe J., Young W.K., Robinson B.H., Steytler D.C. Scattering studies of microemulsions in low-density alkanes density alkanes. J Chem Soc Faraday Trans. 1990;86(16):2883–2889. [Google Scholar]
  • 41.Israelachvili J.N., Mitchell D.J., Ninham B.W. Theory of self-assembly of hydrocarbon amphiphiles into micelles and bilayers. J Chem Soc Faraday Trans 2 – Mol Chem Phys. 1976;72:1525–1568. [Google Scholar]
  • 42.Oakenfull D. Constraints of molecular packing on the size and stability of microemulsion droplets. J Chem Soc Faraday Trans 1. 1980;76:1875–1886. [Google Scholar]
  • 43.Luisi P.L., Magid L.J., Fendler J.H. Solubilization of enzymes and nucleic acids in hydrocarbon micellar solution. Crit Rev Biochem. 1986;20(4):409–474. doi: 10.3109/10409238609081999. [DOI] [PubMed] [Google Scholar]
  • 44.Luisi P.L. Enzyme als Gastmoleküle in inversen Micellen. (1985) Angew Chem. 1985;97:449–460. [Google Scholar]
  • 45.Bellocq A.M. Phase equilibria of polymer-containing microemulsions. Langmuir. 1998;14(14):3730–3739. [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary figures
mmc1.doc (1.7MB, doc)

Articles from Journal of Advanced Research are provided here courtesy of Elsevier

RESOURCES