Abstract
AIMS: To detect the presence of human herpes virus 6 (HHV6) and endogenous biotin in paraffin wax embedded and frozen salivary glands. METHODS: Two stage indirect and streptavidin-biotin immunoperoxidase techniques were used to visualise the antigens. RESULTS: HHV6 could not be shown in any of the tissues. However, considerable endogenous biotin antigenicity was detected in the glandular elements of the paraffin wax embedded material. CONCLUSIONS: Results obtained with avidin-biotin detection systems should be interpreted with caution, especially when glandular epithelium is being stained. This may apply to both immunoperoxidase and in situ hybridisation techniques. The use of an anti-biotin antibody as a standard control should be considered.
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- Fox J. D., Briggs M., Ward P. A., Tedder R. S. Human herpesvirus 6 in salivary glands. Lancet. 1990 Sep 8;336(8715):590–593. doi: 10.1016/0140-6736(90)93392-3. [DOI] [PubMed] [Google Scholar]
- Harnett G. B., Farr T. J., Pietroboni G. R., Bucens M. R. Frequent shedding of human herpesvirus 6 in saliva. J Med Virol. 1990 Feb;30(2):128–130. doi: 10.1002/jmv.1890300209. [DOI] [PubMed] [Google Scholar]
- Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
- Krueger G. R., Wassermann K., De Clerck L. S., Stevens W. J., Bourgeois N., Ablashi D. V., Josephs S. F., Balachandran N. Latent herpesvirus-6 in salivary and bronchial glands. Lancet. 1990 Nov 17;336(8725):1255–1256. doi: 10.1016/0140-6736(90)92874-h. [DOI] [PubMed] [Google Scholar]
- Wood G. S., Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem. 1981 Oct;29(10):1196–1204. doi: 10.1177/29.10.7028859. [DOI] [PubMed] [Google Scholar]