Same sample and imaging conditions as
Figure 4. (
A) Example measurement at the center of the FOV close to the cover glass at the top of the sample. (
B) Mean intensity projections through the XY, XZ, and YZ slices of the bead. (
C) Gaussian fit through the lateral slice through the bead image. (
D) Same as (
C), axial slice. (
E–
H) Same as (
A–
D) for a bead at the bottom of the imaging volume (RF set to 1 mm deep). (
I) Average lateral full-width-at-half-max (FWHM) of the central XY slice of a bead, averaged across the X and Y directions. (
J) Average axial FWHM. (
K) Average normalized brightness of the beads. Error bars, standard deviation.