Skip to main content
. 2016 Feb 22;7(14):18247–18261. doi: 10.18632/oncotarget.7570

Figure 2. HGF induced significantly EMT translation in lung cancer cells.

Figure 2

A549 and 95D cells pretreated with or without 5μM c-Met inhibitors, SU11274, for 1h and then stimulated with 50 ng/mL of HGF for 72 hours. A. The change in morphology was observed under a light microscope. Both these two cells showed mesenchymal features after the HGF treatment, but pretreatment with SU11274 inhibited HGF induced phenomenon of EMT in A549 and 95D cells. Bar = 50μm. B. Western blot analysis showed that E-cadherin expression reduced, and vimentin expression increased in the two cell types after the HGF stimulation. In contrast, SU11274 inhibited the HGF induced vimentin upregulation and E-cadherin downregulation. C, D. Wound healing and transwell invasion assays showed that HGF induced migration and invasion of A549 and 95D cells, and the effects can be attenuated by SU11274 pretreatment. Bar = 200μm. Triplicate assays were performed. C: control; SU: SU11274. ** P<0.01 vs control.