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. 2016 Feb 28;7(14):18356–18370. doi: 10.18632/oncotarget.7786

Figure 3. SH3BP1 facilitated HCC invasion and metastasis via Rac1-WAVE2 pathway.

Figure 3

A. SH3BP1-depleted and Si-Control HCCLM3 cells whole-cell lysates was measured by Western blotting. No signal for WAVE2 was detected in the whole-cell lysates when SH3BP1 depletion but Rac1 specifically suppressed alone by FTY720. B. WAVE2-overexpressed SH3BP1-depleted HCCLM3 transfected with WAVE2 plasmid in Si-SH3BP1 group exhibited behaviors of cell motility and invasion similar to the cells in Si-Control group in vitro (P < 0.05). C. Comparison of SH3BP1, Rac1, and WAVE2 expressions between paired PL and ML tissues (n = 10). Relative mRNA levels of these genes were significantly increased in the ML tissues. Statistical analyses were carried out using a paired Student's t-test, **P < 0.01. D. A positive correlation was found between SH3BP1 expression, Rac1 expression and WAVE2 expression (r = 0.713, P = 0.017; r = 0.816, P = 0.009, respectively). PL: primary lesions; ML: Intrahepatic metastasis lesions.