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. 2016 Feb 23;7(14):18620–18630. doi: 10.18632/oncotarget.7620

Figure 4. Lenti-shRNAs mediated down-regulation of Nek7 inhibited HCC cell growth.

Figure 4

HepG2 cells were infected with lenti-shNek7-1, -2 and negative control lenti-shNC. At 48 h post-transfection, the mRNA and protein levels of Nek7 expression were determined using RT-PCR A. and Western blot B. analyses. β-actin was used as internal control for Western blot and RT-PCR. HepG2 C. and SMMC7721 D. cells were infected with lenti-shNek7-1, -2 and negative control lenti-shNC, respectively. Cell viability was determined using the CCK-8 assay 7 days after infection. Nek7 expression was detected in HCC cells via immunohistochemical staining with anti-Nek7 antibody under a confocal laser scanning microscope. Experiments were repeated three times. (*p < 0.05).