Figure 8. Effects of the Met-DCA cocrystal MetH₂⁺⁺•2DCA⁻ on different molecular signaling pathways.

JVM-2 cells were treated with the Met-DCA cocrystal MetH₂⁺⁺•2DCA⁻(10 mM) for different time-points as indicated. Cell lysates were harvested and analyzed in immunoblotting using specific antibodies to detect: Mcl-1, Bcl-2 A. Akt, phospho-Akt (Ser473), STAT3, phospho-STAT3 (Ser727) B. AMPKα, phospo-AMPKα (Thr172) C. Tubulin staining is shown as a loading control. Representative examples of Western blotting results of three independent experiments are shown.