(a) The SR calculation of
(using optimal fthreshold value) and
(using optimal ΔΔGthreshold value) when taking N>5. (b) The SR calculation of
(using optimal fthreshold value) and
(using optimal ΔΔGthreshold value) when taking N>10. (c) The SR calculation of
(using optimal fthreshold value) and
(using optimal ΔΔGthreshold value) when taking N>5. (d) The SR calculation of
(using optimal fthreshold value) and
(using optimal ΔΔGthreshold value) when taking N>10. N>5 and N>10 means only the SAV, which are observed at least five or ten times in the datasets, will be used for SR calculation.