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. 2016 May 23;65(8):2235–2248. doi: 10.2337/db15-1443

Figure 1.

Figure 1

Eif4ebp2-deficient mice exhibit improved glucose tolerance and β-cell mass expansion. A: Western blotting for 4E-BP1 and 4E-BP2 in wild-type (WT), Eif4ebp1−/−, and Eif4ebp2−/− mice. B and C: Glucose tolerance test (2 g/kg) in wild-type (black circles) and Eif4ebp2−/− mice (gray squares) at 3 and 12 months of age. D: Insulin tolerance test in wild-type and Eif4ebp2−/− mice. Mice were fasted for 6 h before insulin tolerance tests. E: Glucose-stimulated insulin secretion in the wild-type and Eif4ebp2−/− mice. F: Glucose-stimulated insulin secretion determined by static incubation of isolated islets. G: Glucose-induced insulin secretion from isolated islets was measured in perifusion experiments at 2 mmol/L (basal) or with stimulation of KCl (30 mmol/L). The perfusate was collected at 3-min intervals, and insulin concentrations were determined using ELISA. Data are shown as mean ± SEM (n = 7 mice per group). *P < 0.05.