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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Hepatology. 2016 Apr 28;64(2):439–455. doi: 10.1002/hep.28541

Figure 2. Interactions of Matα1 with c-Myc, Mnt, Max, MafG and c-Maf.

Figure 2

(A) The liver protein lysates from control and 14-day LCA treated livers were subjected to IP using anti-Matα1, anti-c-Myc, anti-c-Maf and MafG, followed by mass spectrometry. The table shows a small subset of scores representing potential interacting proteins of interest. NS=no score. The liver protein lysates from control and 14-day LCA were immunoprecipitated (IP) with either antibodies to (B) Matα1, (C) c-Myc, (D) c-Maf, or (E) MafG and then subjected to Western blotting (IB) for Matα1, c-Myc, c-Maf, Maf, Mnt and Max. Histone H3 served as loading control. (F) In vitro pull down assay using immobilized Matα1 (left) or c-Myc (right). Matα1 directly interact with c-Myc, Max, Maf and MafG (left), and c-Myc directly interact with Max, Maf and MafG (right). Results represent a total of at least 3 independent experiments done in duplicate.