Merestinib blocks Mnk kinase activity in acute myeloid leukemia progenitors and exhibits antileukemic effects in vitro and in vivo

Key Points

• Merestinib blocks Mnk kinase activity in acute myeloid leukemia cells.

• Merestinib suppresses human leukemic progenitors and exhibits potent antileukemic effects in a xenograft mouse model.

Abstract

Mitogen-activated protein kinase interacting protein kinases (Mnks) play important roles in the development and progression of acute myeloid leukemia (AML) by regulating eukaryotic translation initiation factor 4E (eIF4E) activation. Inhibiting Mnk1/2-induced phosphorylation of eIF4E may represent a unique approach for the treatment of AML. We provide evidence for antileukemic effects of merestinib, an orally bioavailable multikinase inhibitor with suppressive effects on Mnk activity. Our studies show that merestinib effectively blocks eIF4E phosphorylation in AML cells and suppresses primitive leukemic progenitors from AML patients in vitro and in an AML xenograft model in vivo. Our findings provide evidence for potent preclinical antileukemic properties of merestinib and support its clinical development for the treatment of patients with AML.

Introduction

Aberrant activation of multiple signaling pathways has been implicated in the pathogenesis of acute myeloid leukemia (AML).^1,2 The selective targeting of these pathways could improve the outcome of the currently available, generally unsatisfactory, treatments for patients with AML.^3-5 The mitogen-activated protein kinase (MAPK) pathways regulate multiple cellular processes including leukemic cell proliferation, differentiation, and apoptosis.^1,6 Two key effectors of MAPK pathways are the MAPK interacting protein kinases 1 and 2 (Mnk1/2), which are activated downstream of MAP kinases and regulate the activation of eukaryotic translation initiation factor 4E (eIF4E). eIF4E is a key component of the cap-binding complex required for mRNA translation of mitogenic proteins, including cyclins, c-Myc, and Bcl-xl, and its activity has been linked to leukemogenesis and malignant cell proliferation.^7-9 The phosphorylation and activation of eIF4E by Mnk1/2 on serine 209 (Ser209) is critical for its oncogenic activity.^10,11 As Mnk1/2 double knockout mice have a normal phenotype,¹² Mnk1/2 are attractive targets for cancer therapy as their inhibition could conceivably target selectively malignant cells.

Merestinib, an orally bioavailable small-molecule multikinase inhibitor, suppresses Mnk1/2 activity¹³ and inhibits tumor growth and metastasis in models of non–small lung cancer.^14,15 In this study, we investigated whether merestinib has antileukemic properties. For this purpose, we used in vitro and in vivo models of AML.

Study design

The MV4-11 human leukemia cell line was obtained from ATCC. MM6 cells were purchased from DSMZ. Peripheral blood or bone marrow from patients with AML were collected after obtaining written informed consent as approved by the institutional review board of Northwestern University. Merestinib (LY2801653) was from Eli Lilly and Company (Indianapolis, IN). All animal studies were approved by the Northwestern University Institutional Animal Care and Use Committee. Details about experimental procedures can be found in supplemental Materials and methods, available on the Blood Web site.

Results and Discussion

In initial studies, we examined the effects of merestinib on eIF4E phosphorylation in AML cells. Treatment of MV4-11 (Figure 1A) or MM6 (Figure 1B) cells with merestinib blocked phosphorylation of eIF4E on Ser209. Similarly, merestinib treatment decreased eIF4E phosphorylation on Ser209 in a dose- and time-dependent manner in patient-derived primary AML cells (Figure 1C). Next, to assess whether inhibition of eIF4E phosphorylation results in inhibitory effects on cap-dependent mRNA translation, polysomal fractionation analysis was carried out. Treatment with merestinib resulted in suppression of polysomal peaks (supplemental Figure 1A, left). In addition, merestinib significantly inhibited the polysomal mRNA expression of MCL-1, BCL-2, MYC,and cyclin B1 (CCNB1) in MV4-11 cells (supplemental Figure 1A, right). Consistent with these findings, MCL-1 protein levels were found to be reduced after 4-hour treatment with merestinib (supplemental Figure 1B).

Figure 1.

Merestinib blocks phosphorylation of eIF4E and suppresses growth of primary leukemic progenitors from AML patients. (A) MV4-11 cells, (B) MM6 cells, and (C) AML patient-derived cells (AML-PT) were incubated with merestinib (LY2801653) at final concentrations of either 0.1 or 1 µM for 1 and 4 hours. Cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotted with an antibody against the phosphorylated form of eIF4E on serine 209. The same blots were stripped and reprobed with an antibody against eIF4E. The immunoblots were also probed for HSP90 as a loading control. (D) MV4-11 and (E) MM6 cells were plated in clonogenic assays in methylcellulose with increasing concentrations of merestinib (LY2801653), as indicated. Data are expressed as percentage of colony formation of control vehicle-treated cells, and bar graphs represent means ± standard error (SE) of 5 independent experiments. (F) Dose-dependent suppression of primary leukemic precursors from AML patients by merestinib in clonogenic assays in methylcellulose. Data are expressed as percentage of colony formation of control vehicle-treated cells. Bar graphs represent means ± SE from 5 independent experiments, using cells from 5 different patients with AML. One-way ANOVA analysis followed by Tukey’s test was used to evaluate statistically significant differences: *P < .05, ****P < .0001.

In subsequent studies, merestinib treatment resulted in dose-dependent suppression of cell viability of MV4-11 and MM6 cells in water-soluble tetrazolium salt-1 assays (supplemental Figure 2), suggesting potent antileukemic properties. This prompted further studies, aimed to determine the effects of merestinib on primitive leukemic precursors. Merestinib-treatment resulted in potent inhibition of MV4-11 or MM6-derived leukemic progenitor colony formation (Figure 1D-E). It also resulted in inhibitory effects on primary leukemic progenitors from different patients with AML (Figure 1F). There were also suppressive effects on normal CD34⁺-derived colony-forming unit–granulocyte/macrophage, but these were only statistically significant at higher concentrations (supplemental Figure 3).

To understand the mechanisms by which this agent exhibits antileukemic properties, its effects on cell cycle progression were assessed. Short-term exposure to merestinib blocked cell cycle progression into the G2/M phase (supplemental Figure 4) and inhibited cyclin A2 and cyclin B1 protein expression in AML cells (Figure 2A), consistent with cell cycle arrest. This arrest was followed by leukemic cell apoptosis after long-term merestinib treatment and was associated with continuous suppression of eIF4E phosphorylation (Figure 2B-C; supplemental Figure 5).

Figure 2.

Antileukemic properties of merestinib in vitro and in vivo. (A) Expression of cell cycle markers in merestinib-treated MV4-11 cells. Cells were treated with or without merestinib (10 nM) for the indicated times. Whole cell lysates were evaluated by western blot analysis with the indicated antibodies. (B-C) MV4-11 cells were incubated for 24 and 48 hours in the presence or absence of merestinib (LY2801653) at the indicated doses. Whole cell lysates were analyzed by western blot with the indicated antibodies. (D-F) MM6 cells were injected subcutaneously into the left flank of nu/nu mice (n = 10). Once tumors reached a measurable size, mice were divided into control (vehicle-Captisol) and merestinib (LY2801653) (12 mg/kg)-treated groups. (D) Mice body weight was recorded throughout the study. (E) Average of tumor volumes treated with vehicle or merestinib. Data are means ± SE of tumor volumes. Mann-Whitney test was used to assess statistically significant differences between the 2 treatment groups (*P < .05, **P < .01, ***P < .001). The arrow symbols indicate that mice were killed when significant morbidity was observed as described in the Study design. (E) Kaplan-Meier survival analysis of control and merestinib-treated mice, P = .0006, using a log-rank (Mantel-Cox) test.

To determine whether merestinib exhibits antileukemic properties in vivo, its effects on an AML xenograft model were determined. No statistically significant differences were noted in body weight between vehicle and merestinib-treated mice (Figure 2D). On the other hand, merestinib-treatment potently suppressed AML tumor growth compared with the control group (Figure 2E). Additionally, daily merestinib treatment prolonged survival compared with the vehicle-treated control group (Figure 2F), establishing that this agent exhibits potent antileukemic properties in vivo.

Mnks are important components of MAPK pathways, which are constitutively activated in hematologic malignancies and play key roles in malignant hematopoietic cell survival.^16,17 Mnk-dependent eIF4E phosphorylation has been associated with cancer initiation and metastasis in in vitro and in vivo tumorigenesis models,^10,11 making eIF4E an attractive therapeutic target for the treatment of malignancies.^18-20 We previously reported that cercosporamide, a natural antifungal agent with potent Mnk1/2 inhibitory effects, suppresses Mnk-induced phosphorylation of eIF4E in human AML cell lines and that this correlates with decreased cell proliferation/viability in vitro and in vivo.²¹ Others have shown that ribavirin, an antiviral guanosine analog, reduces eIF4E activity and induces responses in some patients with M4/M5 refractory AML in a clinical trial.^19,22 Together, these studies support that specific targeting of Mnk-dependent eIF4E phosphorylation and/or eIF4E are potentially highly promising approaches for the treatment of AML.

In the present study, we sought to evaluate the antileukemic properties of merestinib, an orally bioavailable agent.¹³ This multikinase inhibitor blocks cell proliferation and tumor growth in multiple in vitro and in vivo cancer models.^13-15 We report that merestinib rapidly and effectively inhibits eIF4E phosphorylation in AML cell lines and in patient-derived cells, leading to suppression of cellular proliferation and cell viability in vitro. Notably, our study demonstrates that merestinib results in negative regulatory effects on mRNA translation of genes encoding mitogenic proteins. We also demonstrate that merestinib blocks cell cycle progression, possibly by impairing the protein expression of key cell cycle regulators in G2 and M phase, such as cyclin B1.²³ Moreover, we provide evidence that inhibition of eIF4E phosphorylation by merestinib is associated with induction of apoptosis and we establish that merestinib exhibits anti-leukemic properties in a human AML xenograft model. It is important to note that merestinib inhibits several other kinases, including FLT3 (FMS-like tyrosine kinase receptor-3) and the MET receptor kinase.¹³ FLT3 activating mutations are expressed in some AML cells,²⁴ including MV4-11 cells that express FLT3-internal tandem duplication (ITD).²⁵ Of the FLT3 mutations, the ITD in AML patients is associated with poor prognosis compared with patients with FLT3 wild-type gene.^25-27 The function of eIF4E can be controlled by 2 major pathways that play a critical role in leukemogeniesis, the MAPK and mammalian target of rapamycin pathways,²⁸ which can be activated downstream of FLT3 receptor.^29,30 Our work demonstrates that inhibition of eIF4E results in antileukemic responses in both FLT3-mutated cell lines and FLT3 WT AML patient-derived cells. In addition, a recent study has implicated activation of MET receptor kinase as a target for the treatment of a subset(s) of AML patients.³¹ It remains to be seen whether this mechanism also contributes to merestinib regulatory effects, possibly by inactivation of MET-dependent engagement of the Mnk/eIF4E pathway. Independently of the precise mechanism, the current report establishes merestinib as a potent Mnk-eIF4E inhibitor with important antileukemic effects in AML progenitors and provides a rationale for clinical studies to assess the effects of this inhibitor in patients with refractory AML.

Authorship

Contribution: E.M.K., D.S., F.E., and L.C.P. designed research; E.M.K., D.S., B.K, E.M.B., F.E., G.T.B., S.M.A., and J.K.A. performed research; E.M.K., D.S., B.K., E.M.B., F.E., F.J.G., and L.C.P. analyzed data/interpreted experimental results; and E.M.K., D.S., F.E., F.J.G., and L.C.P. wrote/edited the manuscript.

Conflict-of-interest disclosure: The authors declare no competing financial interests.

Correspondence: Leonidas C. Platanias, Robert H. Lurie Comprehensive Cancer Center, 303 East Superior St, Lurie 3-125, Chicago, IL 60611; e-mail: l-platanias@northwestern.edu.