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. 1992 Aug 1;89(15):6746–6750. doi: 10.1073/pnas.89.15.6746

Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2.

B Daignan-Fornier 1, G R Fink 1
PMCID: PMC49580  PMID: 1495962

Abstract

We have found cross-pathway regulation between purine and histidine biosynthesis in yeast. The transcription factors BAS1 and BAS2/PHO2, which are also regulators of the histidine pathway, participate in the regulation of the purine biosynthetic pathway. Analysis of four genes of the purine pathway (ADE1, ADE2, ADE5,7, and ADE8) shows that their expression is repressed by adenine. The maximal basal and induced expression of these purine genes requires the presence of both BAS1 and BAS2. The factor BAS1 has been shown to bind at a site containing the TGACTC hexanucleotide motif in the ADE2 and ADE5,7 promoters. This motif is required for both basal and induced activation of the ADE2 gene by BAS1 and BAS2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arndt K. T., Styles C., Fink G. R. Multiple global regulators control HIS4 transcription in yeast. Science. 1987 Aug 21;237(4817):874–880. doi: 10.1126/science.3303332. [DOI] [PubMed] [Google Scholar]
  2. Arndt K., Fink G. R. GCN4 protein, a positive transcription factor in yeast, binds general control promoters at all 5' TGACTC 3' sequences. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8516–8520. doi: 10.1073/pnas.83.22.8516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Braus G., Mösch H. U., Vogel K., Hinnen A., Hütter R. Interpathway regulation of the TRP4 gene of yeast. EMBO J. 1989 Mar;8(3):939–945. doi: 10.1002/j.1460-2075.1989.tb03455.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crowley J. C., Kaback D. B. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation of the ADE1 gene. J Bacteriol. 1984 Jul;159(1):413–417. doi: 10.1128/jb.159.1.413-417.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gianì S., Manoni M., Breviario D. Cloning and transcriptional analysis of the ADE6 gene of Saccharomyces cerevisiae. Gene. 1991 Oct 30;107(1):149–154. doi: 10.1016/0378-1119(91)90309-y. [DOI] [PubMed] [Google Scholar]
  6. Henikoff S. The Saccharomyces cerevisiae ADE5,7 protein is homologous to overlapping Drosophila melanogaster Gart polypeptides. J Mol Biol. 1986 Aug 20;190(4):519–528. doi: 10.1016/0022-2836(86)90238-x. [DOI] [PubMed] [Google Scholar]
  7. Hinnebusch A. G. Mechanisms of gene regulation in the general control of amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol Rev. 1988 Jun;52(2):248–273. doi: 10.1128/mr.52.2.248-273.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miasnikov A. N., Plavnik Iu A., Sasnauskas K. V., Gedminene G. K., Ianulaitis A. A. Nukleotidnaia posledovatel'nost' gena ADE 1 drozhzhei Saccharomyces cerevisiae. Bioorg Khim. 1986 Apr;12(4):555–558. [PubMed] [Google Scholar]
  9. Myers A. M., Tzagoloff A., Kinney D. M., Lusty C. J. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene. 1986;45(3):299–310. doi: 10.1016/0378-1119(86)90028-4. [DOI] [PubMed] [Google Scholar]
  10. Mäntsälä P., Zalkin H. Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase. J Biol Chem. 1984 Jul 10;259(13):8478–8484. [PubMed] [Google Scholar]
  11. Mösch H. U., Scheier B., Lahti R., Mäntsäla P., Braus G. H. Transcriptional activation of yeast nucleotide biosynthetic gene ADE4 by GCN4. J Biol Chem. 1991 Oct 25;266(30):20453–20456. [PubMed] [Google Scholar]
  12. Nagawa F., Fink G. R. The relationship between the "TATA" sequence and transcription initiation sites at the HIS4 gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8557–8561. doi: 10.1073/pnas.82.24.8557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Staben C., Rabinowitz J. C. Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase. J Biol Chem. 1986 Apr 5;261(10):4629–4637. [PubMed] [Google Scholar]
  14. Stotz A., Linder P. The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors. Gene. 1990 Oct 30;95(1):91–98. doi: 10.1016/0378-1119(90)90418-q. [DOI] [PubMed] [Google Scholar]
  15. Tice-Baldwin K., Fink G. R., Arndt K. T. BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2. Science. 1989 Nov 17;246(4932):931–935. doi: 10.1126/science.2683089. [DOI] [PubMed] [Google Scholar]
  16. White J. H., DiMartino J. F., Anderson R. W., Lusnak K., Hilbert D., Fogel S. A DNA sequence conferring high postmeiotic segregation frequency to heterozygous deletions in Saccharomyces cerevisiae is related to sequences associated with eucaryotic recombination hotspots. Mol Cell Biol. 1988 Mar;8(3):1253–1258. doi: 10.1128/mcb.8.3.1253. [DOI] [PMC free article] [PubMed] [Google Scholar]

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