FIG 6 .

Cig2 function decreases host tolerance of C. burnetii infection. (A) Images of hemocytes isolated from G. mellonella larvae that were infected with either the parental strain of C. burnetii producing GFP or an isogenic cig2::Tn mutant strain. Images show C. burnetii (green), actin (red), and DNA (DAPI). Bars, 10 µm. (B and C) Curves indicate the percentage of surviving G. mellonella larvae at each day after infection with the indicated strains of C. burnetii. “cig2::Tn (A)” and “cig2::Tn (B)” denote two independent cig2::Tn mutants. Data represent the averages of the results of three independent experiments in which an individual strain of C. burnetii was used to infect 15 larvae. (D) C. burnetii numbers were determined by measuring genome equivalents in samples from infected G. mellonella larvae at the time of death. Each separate symbol represents data from an infected individual. Data were averaged from results of two independent experiments. (E) C. burnetii numbers were determined by measuring genome equivalents in larvae each day after infection. Each symbol represents C. burnetii numbers obtained from an individual larva.