TABLE 4 .

Specific carnitine acetyltransferase activities in cell extracts of S. cerevisiae strains^a

Strain	Short descriptionb	Carbon source in the medium	Carnitine acetyltransferase activity (µmol ⋅ mg protein−1 ⋅ min−1)c
IMX585	Reference strain	Glucose	BD
IMX868	{CARN}	Glucose	2.69 ± 0.51
IMX923	sga1Δ::pADH1-YAT2	Glucose	BD
IMX925	sga1Δ::pADH1-YAT2C173G	Glucose	BD
IME140	Empty multicopy plasmid	Glucose	BD
IME320	Multicopy plasmid pADH1-YAT2	Glucose	BD
IME321	Multicopy plasmid pADH1-YAT2C173G	Glucose	BD
IME233	Multicopy plasmid pTDH3-CAT2	Glucose	4.24 ± 0.52
CEN.PK113-7D	CAT2 YAT1 YAT2	Ethanol	1.75 ± 0.02
CEN.PK215-4A	cat2Δ yat1Δ YAT2	Ethanol	BD
IMX745	{CARN}	Glucose	3.19 ± 0.14
IMS0482	{CARN} evolution line 1	Glucose	2.39 ± 0.05
IMX852	{CARN,pADH1-YAT2} MCT1T641GRTG2G503T	Glucose	2.92 ± 0.73
IMX913	{CARN,pADH1-YAT2C173G} MCT1T641G RTG2G503T	Glucose	3.11 ± 0.71
IMX932	{CARN,yat2Δ} MCT1T641G RTG2G503T	Glucose	2.82 ± 0.44

^aStrains were grown in shake flasks containing synthetic medium with either 20 g ⋅ liter⁻¹ glucose or 2% (vol/vol) ethanol as the carbon source and harvested in mid-exponential phase.

^bThe composition of the {CARN} gene set is described in Materials and Methods.

^cCarnitine acetyltransferase activities in cell extracts were obtained from duplicate growth experiments and are shown as means ± standard deviations. The detection limit of the enzyme assay was 0.01 µmol ⋅  mg protein⁻¹ ⋅ min⁻¹. BD, below detection.