Rhoptry kinome molecules are essential for chronic infection. C57BL/6 mice were infected i.p. with 200 tachyzoites of each strain, and brain cyst burdens were measured 21 days postinfection. Data shown are cumulative results from one to three independent experiments for each strain tested. ROPK knockout strains and the Δku80 parent strain are organized in descending order by cyst number for each strain, with cyst numbers shown as means plus standard errors of the means (SEM) (error bars). P values were calculated by a Student’s t test, and a P < 0.05 (shown in purple or red color) was considered significant. Data on the ROPK knockout strains and the Δku80 parent strain follow: Δrop16 (two experiments; n = 6 mice; P = 0.0242), Δku80 parent (three experiments; n = 12 mice), Δrop42/43/44 (one experiment; n = 4 mice; P = 0.7188), Δrop39 (two experiments; n = 7 mice; P = 0.5691), Δrop23 (one experiment; n = 4 mice; P = 0.5232), Δrop45 (one experiment; n = 4 mice; P = 0.4186), Δrop24 (one experiment; n = 4 mice; P = 0.4259), Δrop32 (one experiment; n = 4 mice; P = 0.1651), Δrop11 (two experiments; n = 7 mice; P = 0.0703), Δrop20 (two experiments; n = 6 mice; P = 0.0534), Δrop28 (one experiment; n = 4 mice; P = 0.0928), Δrop26 (two experiments; n = 5 mice; P = 0.0500), Δrop41 (two experiments; n = 5 mice; P = 0.0384), Δrop37 (two experiments; n = 5 mice; P = 0.0154), Δrop4/7 (three experiments; n = 12 mice; P = 0.0198), Δrop36 (two experiments; n = 6 mice; P = 0.0070), Δrop31 (two experiments; n = 6 mice; P = 0.0058), Δrop21 (two experiments; n = 7 mice; P = 0.0036), Δrop40 (one experiment; n = 4 mice; P = 0.0217), Δrop27 (two experiments; n = 6 mice; P = 0.0073), Δrop25 (two experiments; n = 7 mice; P = 0.0013), Δrop22 (two experiments; n = 5 mice; P = 0.0095), Δrop2/8 (one experiment; n = 4 mice; P = 0.0063), Δrop38/29/19 (two experiments; n = 8 mice; P < 0.0001), Δrop35 (two experiments; n = 8 mice; P < 0.0001), Δrop18 (two experiments; n = 8 mice; P < 0.0001), Δrop17 (two experiments; n = 8 mice; P < 0.0001), and Δrop5 (three experiments; n = 9 mice; P < 0.0001).