. 2014 Dec 18;68(4):809–820. doi: 10.1007/s10616-014-9832-y

Table 1.

List of the differentially expressed protein spots identified by MALDI-TOF MS

Spot No.	NCBI accession	Protein name	pI^a	MW^b	AA^c	Seq Cov (%)^d	Ratio^e Treated:C
1	gi\|7019485	Programmed cell death protein 6	5.16	21,912	191	35	0.13 ± 0.010
2	gi\|338043	SF2p32	4.74	31,287	278	10	0.25 ± 0.018
3	gi\|338043	SF2p32	4.74	31,287	278	10	0.48 ± 0.051
4	gi\|75070716	Heterogeneous nuclear ribonucleoprotein C	4.95	33,670	306	2	0.33 ± 0.021
5	gi\|263657	Cytidine deaminase	5.84	16,556	146	35	0.20 ± 0.018
6	gi\|4929697	CGI-114 protein	5.60	23,967	205	7	0.47 ± 0.047
7	gi\|5453790	Nicotinamide N-methyltransferase	5.56	30,011	264	12	0.08 ± 0.004
8	gi\|4506667	60S acidic ribosomal protein P0	5.71	34,423	317	5	0.24 ± 0.013
9	gi\|3646128	Thioredoxin-like protein	5.25	37,751	335	8	0.32 ± 0.028
10	gi\|61212932	ATP-dependent RNA helicase DDX39A	5.46	49,129	427	10	0.27 ± 0.0211
11	gi\|48762932	T-complex protein 1 subunit theta	5.78	54,207	497	9	0.19 ± 0.009
12	gi\|1008915	Proteasome activator hPA28 subunit beta	5.44	27,502	239	24	0.27 ± 0.023
13	gi\|1060907	Quinolinate phosphoribosyltransferase	5.81	31,232	297	7	0.39 ± 0.025
14	gi\|332356380	Albumin	5.73	66,531	585	13	0.43 ± 0.034
15	gi\|49256408	Eukaryotic translation initiation factor 4B	5.54	69,166	611	9	0.37 ± 0.031
16	gi\|312137	Fructose bisphosphate aldolase	6.41	39,816	364	18	0.46 ± 0.056
17	gi\|728777	Replication factor C subunit 5	6.72	38,496	340	6	0.46 ± 0.021
18	gi\|18379349	Synaptic vesicle membrane protein VAT-1 homolog	5.88	42,122	483	12	0.49 ± 0.036
19	gi\|1172837	Ran-specific GTPase-activating protein	5.19	23,310	201	3	3.61 ± 0.059
20	gi\|310943005	Chain A, Crystal Structure Analysis of R29mE81 M Double Mutant of Human CLIC1	5.07	26,899	241	10	2.76 ± 0.054
21	gi\|5453559	ATP synthase subunit d, mitochondrial isoform a	5.21	18,537	161	34	2.97 ± 0.031
22	gi\|4503545	Eukaryotic translation initiation factor 5A-1 isoform B	5.08	17,049	154	14	4.22 ± 0.086
23	gi\|4507669	Translationally-controlled tumor protein	4.84	19,697	172	22	2.94 ± 0.011
24	gi\|3041660	Cyclin-dependent kinase 4 inhibitor A	5.52	16,532	156	14	4.66 ± 0.071
25	gi\|28201890	RuvB-like 2	5.49	51,156	463	11	2..56 ± 0.011
26	gi\|189617	Protein PP4-X	5.65	36,262	321	17	2.14 ± 0.013
27	gi\|62897507	Isocitrate dehydrogenase 3(NAD +) alpha precursor variant	6.47	40,050	366	10	2.19 ± 0.006
28	gi\|125962	Lamin-A/C	6.57	74,139	664	23	2.83 ± 0.023
29	gi\|313104306	Far upstream element-binding Protein 2	6.26	73,146	711	8	3.02 ± 0.009
30	gi\|115311883	Methyltransferase-like protein 19	6.98	84,628	757	17	2.32 ± 0.011
31	gi\|38455427	T-complex protein 1 subunit delta	7.96	58,401	539	9	2.41 ± 0.013
32	gi\|7387554	Mitotic checkpoint protein BUB3	6.36	37,154	328	3	2.12 ± 0.024
33	gi\|119597758	dUTP pyrophosphatase, isoform CRA_d	9.88	23,042	218	13	3.08 ± 0.071
34	gi\|4506517	Regulator of G-protein signaling 2	9.05	24,381	211	5	2.76 ± 0.019
35	gi\|4758788	NADH dehydrogenase[ubiquinone] iron-sulfur protein 3, mitochondrial precursor	6.89	30241	264	35	2.32 ± 0.010

^a Isoelectric point. ^b Molecular weight. ^c Amino acid. ^d Sequence coverage (%). ^e The spot intensity ratios were calculated for HU-treated (treated) to non-treated control (C)