Table 1.
Gene | Primer sequence (5′–3′) | Product size | Accession number |
---|---|---|---|
Klf2 | F: AAGACCTACACCAAGAGTTCGCATC R: TCTGAGCGCGCAAACTTCC |
110 | NM_016270.2 |
Klf5 | F: ACGCATCCACTACTGCGATTACC R: AACCTCCAGTCGCAGCCTTC |
132 | NM_001730.3 |
Klf4 | F: AAGAGTTCCCATCTCAAGGCACA R:GGGCGAATTTCCATCCACAG |
91 | NM_004235.4 |
Oct4 | F: CTTGCTGCAGAAGTGGGTGGAGGAA R: CTGCAGTGTGGGTTTCGGGCA |
169 | NM_001159542.1 |
Nanog | F: GAACTCTCCAACATCCTGAACCTC R: CCTTCTGCGTCACACCATTGC |
127 | NM_024865.2 |
Rex1 | F: GGAATGTGGGAAAGCGTTCGT R: CCGTGTGGATGCGCACGT |
152 | NM_174900.3 |
ON | F: CGAGCTGGATGAGAACAACA R: AAGTGGCAGGAAGAGTCGAA |
126 | BC072457 |
COMP | F: AGGGAGATCGTGCAGACAA R: AGCTGGAGCTGTCCTGGTAG |
154 | NM_000095 |
LPL | F: AGAGCCAAAAGAAGCAG R: GGCAGAGTGAATGGGAT |
182 | NM_000237 |
β-actin | F: CACACTGTGCCCATCTACGA R: TGAGGATCTTCATGAGGTAGTCAG |
106 | GZ549052 |