Fig. 2.

Effect of SMYD3 knockdown on OCT4, NANOG and CDX2 expression. a Effect of SMYD3 knockdown in bovine oocytes by SMYD3 siRNA interference. Suppression of SMYD3 was examined at 24 h after GV oocytes injected with SMYD3 siRNA. The non-injection control and nonsence siRNA injection group were used as control. Error bars represent standard deviation (SD) (n = 3). *P < 0.01. b Graphs showing the relative mRNA transcript level of OCT4, NANOG, CDX2 and SMYD3 relative to GAPDH are shown for metaphase II oocytes and embryos 48 h after in vitro fertilization (pIVF) which were treated with siRNA. Error bars represent standard deviation (SD) (n = 3)