Table 4.
LOD (limit of detection) evaluation of Vero cell line infected with mycoplasma (positive control cell line)
| Vero cell line mycoplasma positive | Reading A | Reading B | B/A | Test results |
|---|---|---|---|---|
| Undiluted positive control Vero cell line | 9/514 | 267/860 | 28/154 | Positive > 1 |
| Dilution 1/2 | 13/673 | 132/044 | 9/657 | Positive > 1 |
| Dilution 1/4 | 17/407 | 95/361 | 5/478 | Positive > 1 |
| Dilution 1/8 | 11/005 | 74//011 | 6/725 | Positive > 1 |
| Dilution 1/16 | 7/262 | 48/519 | 6/681 | Positive > 1 |
| Dilution 1/32 | 5/025 | 15/477 | 3/080 | Positive > 1 |
| Dilution 1/64 | 5/555 | 14/522 | 2/614 | Positive > 1 |
| Dilution 1/128 | 3/660 | 7/194 | 1/956 | Positive > 1 |
| Dilution 1/256 | 3/119 | 4/375 | 1/402 | Positive > 1 |
| Dilution 1/512 | 3/390 | 2/685 | 0/792 | Negative < 1 |
| Dilution 1/1024 | 4/686 | 2/121 | 0/452 | Negative < 1 |
| Dilution 1/2048 | 2/279 | 0/931 | 0/408 | Negative < 1 |
| Dilution 1/4096 | 1/892 | 0/682 | 0/360 | Negative < 1 |
| Control positive kit | 6/051 | 216/435 | 35/768 | Positive > 1 |
| Control negative kit | 3/075 | 0/459 | 0/149 | Negative < 1 |
| Vero cell line mycoplasma free (negative) | 2/235 | 0/585 | 0/261 | Negative < 1 |
Different serial dilutions in the range of 1/2 to 1/4,096 were prepared