PBS |
Phosphate buffered saline |
Buffer solution |
PFA |
Paraformaldehyde |
Used to |
BrdU |
Bromodeoxyuridine |
Synthetic nucleoside, analog of thymidine used in the detection of proliferating cells in living tissues. |
OCT |
Optimal cutting temperature compound |
This product is a formulation of water‐soluble glycols and resins and provides optimal conditions for cryostat sectioning at temperatures of −10 C (14 F) and below. |
DAPI |
(4′,6‐diamidino‐2‐phenylindole) |
Fluorescent label that binds to DNA, used to cell staining and visualization via fluorescent microscopy. |
MCM2 |
Minichromosome maintenance complex |
Part of a set of highly conserved proteins involved in the initiation of eukaryotic genome replication. |
Ki67 |
Also known as MKi67 |
Nuclear protein associated with cell proliferation, and with rRNA transcription. Is active during all active phases of cell cycle. |
Iba1 |
Ionized calcium‐binding adapter molecule 1 |
A calcium binding protein, specifically expressed in microglial cells. |