Table 2.

Multiplex PCR primers for vancomycin-resistance and virulence-markers detection in river water isolates of E. faecalis

Multiplexed/singleplexed genes	Oligomer sequence (5′ → 3′)	Oligo-mer conc.a (µM)	Product size (bp)	Reference
sodA (E.faecalis)	F: 5′-ACTTATGTGACTAACTTAACC-3′ R: 5′-TAATGGTGAATCTTGGTTTGG-3′	0.15	360	Jackson et al. (2004)
vanB	F: 5′-CAAAGCTCCGCAGCTTGCATG-3′ R: 5′-TGCATCCAAGCACCCGATATAC-3′	0.20	484	Dahl et al. (1999)
gelE	F: 5′-ACCCCGTATCATTGGTTT-3′ R: 5′-ACGCATTGCTTTTCCATC-3′	0.25	419	Eaton and Gasson (2001)
ace b	F: 5′-GGAATGACCGAGAACGATGGC-3′ R: 5′-GCTTGATGTTGGCCTGCTTCCG-3′	0.29	616	Creti et al. (2004)
efaA b	F: 5′-GCCAATTGGGACAGACCCTC-3′ R: 5′-CGCCTTCTGTTCCTTCTTTGGC-3′	0.30	688	Creti et al. (2004)
esp c	F: 5′-TTGCTAATGCTAGTCCACGACC-3′ R: 5′-GCGTCAACACTTGCATTGCCGAA-3′	0.15	933	Eaton and Gasson (2001)

^aOligomer concentration optimized for this study

^bMultiplexed genes include either ace or efaA gene with sodA, vanB and gelE genes

^cSingleplexed gene