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. Author manuscript; available in PMC: 2016 Jul 28.
Published in final edited form as: Biomacromolecules. 2015 Sep 14;16(10):3172–3179. doi: 10.1021/acs.biomac.5b00795

Figure 4.

Figure 4

Relative NOTCH1 expression levels in PBMCs and their corresponding viabilities (cell density = 1x106 cells/mL). Cells were treated with PTDM/NOTCH1 siRNA complexes or PTDM/scrambled siRNA with an N:P ratio = 8:1 in complete media for four hours at 37°C. After treatment, cells were washed and then stimulated with plate-bound anti-CD3 and anti-CD28 for 48 hours. All data was normalized to an untreated control (grey bar). A) Relative NOTCH1 levels in PBMCs after 48-hour treatment with PTDM/NOTCH1 siRNA (light blue bars) or PTDM/scrambled siRNA complexes (purple bars). B) Percent viable cells following staining with 7-AAD. Data represents the mean ± SEM of four independent experiments using cells isolated from different donors. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ns = not significant, as calculated by the unpaired two-tailed student t-test.