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. 1991 Nov;44(11):900–904. doi: 10.1136/jcp.44.11.900

Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue.

E P Arnoldus 1, E J Dreef 1, I A Noordermeer 1, M M Verheggen 1, R F Thierry 1, A C Peters 1, C J Cornelisse 1, M Van der Ploeg 1, A K Raap 1
PMCID: PMC496626  PMID: 1752979

Abstract

The feasibility was studied of in situ hybridisation using chromosome specific DNA probes on paraffin wax embedded normal and malignant tissues from different organs. Both isolated nuclei and 5 microns sections were used in in situ hybridisation experiments with biotinylated repetitive DNA probes specific for the centromeric regions of chromosomes 1 and 17. The hybridisation results were visualised with peroxidase-diaminobenzidine. The optimal pretreatments with sodium thiocyanate and pepsin were experimentally defined for the different tissues. Although interphase cytogenetics on paraffin wax embedded tissue is possible, the results indicate that it has its limitations, compared with investigations on fresh tumour tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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