Abstract
A new monoclonal antibody Ber-MAC3 is reported. It recognises a formol sensitive epitope of a not yet clustered monocyte/macrophage specific 140 kilodalton glycoprotein that is expressed on the cell surface and in the cytoplasm. In 30 cases of acute and chronic leukaemia, Ber-MAC3 staining was restricted to 15 myeloid leukaemias of M4 and M5 types. The tumour cells of two cases of true histiocytic malignancies were Ber-MAC3 positive, whereas those of all 280 malignancies of lymphocytic origin were negative. The latter included 52 cases of Hodgkin's disease and 41 cases of Ki-1 positive anaplastic large cell lymphomas which had previously been classified as true histiocytic lymphomas. Ber-MAC3 therefore seems to be of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and seems to be suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin.
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Selected References
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- Athanasou N. A., Hall P. A., d'Ardenne A. J., Quinn J., McGee J. O. A monoclonal antibody (anti-L-35) which reacts with human osteoclasts and cells of the mononuclear phagocyte system. Br J Exp Pathol. 1988 Apr;69(2):309–319. [PMC free article] [PubMed] [Google Scholar]
- Bhoopat L., Turner R. R., Stathopoulos E., Meyer P. R., Taylor C. R., Marder R. J., Epstein A. L. Immunohistochemical characterization of two new monoclonal antibodies (LN-4, LN-5) reactive with human macrophage subsets and derived malignancies in B5-fixed, paraffin-embedded tissues. Blood. 1988 Apr;71(4):1079–1085. [PubMed] [Google Scholar]
- Breard J., Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol. 1980 Apr;124(4):1943–1948. [PubMed] [Google Scholar]
- Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
- Cuomo M., Santoro L., Mottolese M., Tecce R., Rosato V., Alescio T., Siccardi A. G., Natali P. G. A murine monoclonal antibody strictly reactive with human peripheral blood monocytes. Clin Immunol Immunopathol. 1987 Dec;45(3):310–322. doi: 10.1016/0090-1229(87)90084-5. [DOI] [PubMed] [Google Scholar]
- Dallenbach F. E., Stein H. Expression of T-cell-receptor beta chain in Reed-Sternberg cells. Lancet. 1989 Oct 7;2(8667):828–830. doi: 10.1016/s0140-6736(89)92997-8. [DOI] [PubMed] [Google Scholar]
- Davey F. R., Cordell J. L., Erber W. N., Pulford K. A., Gatter K. C., Mason D. Y. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988 Jul;41(7):753–758. doi: 10.1136/jcp.41.7.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diehl V., Kirchner H. H., Burrichter H., Stein H., Fonatsch C., Gerdes J., Schaadt M., Heit W., Uchanska-Ziegler B., Ziegler A. Characteristics of Hodgkin's disease-derived cell lines. Cancer Treat Rep. 1982 Apr;66(4):615–632. [PubMed] [Google Scholar]
- Dimitriu-Bona A., Burmester G. R., Waters S. J., Winchester R. J. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983 Jan;130(1):145–152. [PubMed] [Google Scholar]
- Dippold W. G., Klingel R., Bernhard H., Dienes H. P., Knuth A., Meyer zum Büschenfelde K. H. Secretory epithelial cell marker on gastrointestinal tumors and in human secretions defined by a monoclonal antibody. Cancer Res. 1987 Apr 15;47(8):2092–2097. [PubMed] [Google Scholar]
- Flavell D. J., Jones D. B., Wright D. H. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem. 1987 Nov;35(11):1217–1226. doi: 10.1177/35.11.3309045. [DOI] [PubMed] [Google Scholar]
- Gahmberg C. G., Andersson L. C. Selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride. J Biol Chem. 1977 Aug 25;252(16):5888–5894. [PubMed] [Google Scholar]
- Gerdes J., Lemke H., Baisch H., Wacker H. H., Schwab U., Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710–1715. [PubMed] [Google Scholar]
- Herbst H., Tippelmann G., Anagnostopoulos I., Gerdes J., Schwarting R., Boehm T., Pileri S., Jones D. B., Stein H. Immunoglobulin and T-cell receptor gene rearrangements in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma: dissociation between phenotype and genotype. Leuk Res. 1989;13(2):103–116. doi: 10.1016/0145-2126(89)90134-3. [DOI] [PubMed] [Google Scholar]
- Hogg N., MacDonald S., Slusarenko M., Beverley P. C. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology. 1984 Dec;53(4):753–767. [PMC free article] [PubMed] [Google Scholar]
- Hogg N., Selvendran Y. An anti-human monocyte/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue. Cell Immunol. 1985 May;92(2):247–253. doi: 10.1016/0008-8749(85)90006-1. [DOI] [PubMed] [Google Scholar]
- Hogg N., Takacs L., Palmer D. G., Selvendran Y., Allen C. The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar;16(3):240–248. doi: 10.1002/eji.1830160306. [DOI] [PubMed] [Google Scholar]
- Jones D. B., Scott C. S., Wright D. H., Stein H., Beverley P. C., Payne S. V., Crawford D. H. Phenotypic analysis of an established cell line derived from a patient with Hodgkin's disease (HD). Hematol Oncol. 1985 Apr-Jun;3(2):133–145. doi: 10.1002/hon.2900030205. [DOI] [PubMed] [Google Scholar]
- Kamesaki H., Fukuhara S., Tatsumi E., Uchino H., Yamabe H., Miwa H., Shirakawa S., Hatanaka M., Honjo T. Cytochemical, immunologic, chromosomal, and molecular genetic analysis of a novel cell line derived from Hodgkin's disease. Blood. 1986 Jul;68(1):285–292. [PubMed] [Google Scholar]
- Kaplan H. S., Gartner S. "Sternberg-reed" giant cells of Hodgkin's Disease: cultivation in vitro, heterotransplantation, and characterization as neoplastic macrophages. Int J Cancer. 1977 Apr 15;19(4):511–525. doi: 10.1002/ijc.2910190412. [DOI] [PubMed] [Google Scholar]
- Kato T., Wokalek H., Schöpf E., Eggert H., Ernst M., Rietschel E. T., Fischer H. Measurement of chemiluminescence in freshly drawn human blood. I. Role of granulocytes, platelets, and plasma factors in zymosan-induced chemiluminescence. Klin Wochenschr. 1981 Mar 2;59(5):203–221. doi: 10.1007/BF01476577. [DOI] [PubMed] [Google Scholar]
- Kelly P. M., Bliss E., Morton J. A., Burns J., McGee J. O. Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol. 1988 May;41(5):510–515. doi: 10.1136/jcp.41.5.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kreipe H., Radzun H. J., Parwaresch M. R., Haislip A., Hansmann M. L. Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human. J Histochem Cytochem. 1987 Oct;35(10):1117–1126. doi: 10.1177/35.10.3476670. [DOI] [PubMed] [Google Scholar]
- Lukes R. J., Butler J. J. The pathology and nomenclature of Hodgkin's disease. Cancer Res. 1966 Jun;26(6):1063–1083. [PubMed] [Google Scholar]
- MacDonald S. M., Pulford K., Falini B., Micklem K., Mason D. Y. A monoclonal antibody recognizing the p150/95 leucocyte differentiation antigen. Immunology. 1986 Nov;59(3):427–431. [PMC free article] [PubMed] [Google Scholar]
- McCulloch E. A. Stem cells in normal and leukemic hemopoiesis (Henry Stratton Lecture, 1982). Blood. 1983 Jul;62(1):1–13. [PubMed] [Google Scholar]
- Munro C. S., Campbell D. A., Collings L. A., Poulter L. W. Monoclonal antibodies distinguish macrophages and epithelioid cells in sarcoidosis and leprosy. Clin Exp Immunol. 1987 May;68(2):282–287. [PMC free article] [PubMed] [Google Scholar]
- Naiem M., Gerdes J., Abdulaziz Z., Stein H., Mason D. Y. Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistological analysis of lymphoid tissue. J Clin Pathol. 1983 Feb;36(2):167–175. doi: 10.1136/jcp.36.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parwaresch M. R., Radzun H. J., Kreipe H., Hansmann M. L., Barth J. Monocyte/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen. Am J Pathol. 1986 Oct;125(1):141–151. [PMC free article] [PubMed] [Google Scholar]
- Pulford K. A., Rigney E. M., Micklem K. J., Jones M., Stross W. P., Gatter K. C., Mason D. Y. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol. 1989 Apr;42(4):414–421. doi: 10.1136/jcp.42.4.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Radzun H. J. Differenzierungslinien im Monozyten/Makrophagensystem. Verh Dtsch Ges Pathol. 1988;72:50–56. [PubMed] [Google Scholar]
- Radzun H. J., Kreipe H., Bödewadt S., Hansmann M. L., Barth J., Parwaresch M. R. Ki-M8 monoclonal antibody reactive with an intracytoplasmic antigen of monocyte/macrophage lineage. Blood. 1987 May;69(5):1320–1327. [PubMed] [Google Scholar]
- Radzun H. J., Parwaresch M. R., Feller A. C., Hansmann M. L. Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells. Am J Pathol. 1984 Dec;117(3):441–450. [PMC free article] [PubMed] [Google Scholar]
- Radzun H. J., Parwaresch M. R., Sundström C., Nilsson K., Eissner M. Monocytic origin of the human hematopoietic cell line U-937 and its convertibility to macrophages evidenced by isoenzyme mapping. Int J Cancer. 1983 Feb 15;31(2):181–186. doi: 10.1002/ijc.2910310208. [DOI] [PubMed] [Google Scholar]
- Schaadt M., Diehl V., Stein H., Fonatsch C., Kirchner H. H. Two neoplastic cell lines with unique features derived from Hodgkin's disease. Int J Cancer. 1980 Dec 15;26(6):723–731. doi: 10.1002/ijc.2910260605. [DOI] [PubMed] [Google Scholar]
- Schwarting R., Stein H., Wang C. Y. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985 Apr;65(4):974–983. [PubMed] [Google Scholar]
- Seymour G. J., Poulter L. W., Bofill M., Hobbs S., Janossy G., Brooks D., Zola H., Bradley J. The reactivity of a monoclonal antibody against cells of the monocyte-macrophage series in sections of normal and inflamed human tissues. J Reticuloendothel Soc. 1983 Dec;34(6):463–473. [PubMed] [Google Scholar]
- Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
- Stein H., Gerdes J., Schwab U., Lemke H., Diehl V., Mason D. Y., Bartels H., Ziegler A. Evidence for the detection of the normal counterpart of Hodgkin and Sternberg-Reed cells. Hematol Oncol. 1983 Jan-Mar;1(1):21–29. doi: 10.1002/hon.2900010105. [DOI] [PubMed] [Google Scholar]
- Stein H., Mason D. Y., Gerdes J., O'Connor N., Wainscoat J., Pallesen G., Gatter K., Falini B., Delsol G., Lemke H. The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood. 1985 Oct;66(4):848–858. [PubMed] [Google Scholar]
- Todd R. F., 3rd, Nadler L. M., Schlossman S. F. Antigens on human monocytes identified by monoclonal antibodies. J Immunol. 1981 Apr;126(4):1435–1442. [PubMed] [Google Scholar]
- Tsuchiya S., Kobayashi Y., Goto Y., Okumura H., Nakae S., Konno T., Tada K. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 1982 Apr;42(4):1530–1536. [PubMed] [Google Scholar]
- Tsuchiya S., Yamabe M., Yamaguchi Y., Kobayashi Y., Konno T., Tada K. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 1980 Aug;26(2):171–176. doi: 10.1002/ijc.2910260208. [DOI] [PubMed] [Google Scholar]
- Van Voorhis W. C., Steinman R. M., Hair L. S., Luban J., Witmer M. D., Koide S., Cohn Z. A. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J Exp Med. 1983 Jul 1;158(1):126–145. doi: 10.1084/jem.158.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zwadlo G., Bröcker E. B., von Bassewitz D. B., Feige U., Sorg C. A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol. 1985 Mar;134(3):1487–1492. [PubMed] [Google Scholar]
- Zwadlo G., Schlegel R., Sorg C. A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J Immunol. 1986 Jul 15;137(2):512–518. [PubMed] [Google Scholar]