Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 22;8(1):129–140. doi: 10.1080/19420862.2015.1109757

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 1. — Staining method optimization using a dengue-specific hybridoma cell line, 4G2 (A) Titration of DEN-2–80E biotin for use in FACS staining. Final concentration of DEN-2–80E biotin and corresponding geomean fluorescent intensity (MFI) of the PE signal from the 4G2 pan dengue hybridoma (filled circles) and negative control Staphylococcus aureus antigen-specific hybridoma, UKNKC (open circles). (B) DEN-2–80E SA-PE staining identifies antibody secreting cells comparably to an IgG-specific stain. 4G2 hybridoma (transparent histogram), was stained with DEN-2–80E PE (right) or for the hybridoma subtype, IgG2a (left). For comparison, an IgG-1 type Staphylococcus aureus specific hybridoma (filled histogram) is overlayed, (right). (C) Effects of 100X concentration unlabeled DEN-2 80E pre-incubation on DEN-2–80E PE staining. 4G2 hybridomas were stained with 1.6 μg/mL of DEN-2–80E following pre-incubation with (right) or without (left) of 160 μg/mL of unlabeled DEN-2–80E.