Figure 5.

HLA-DR–associated peptides produced by 5 × 10⁶ dendritic cells from 35 different donors exposed separately to G1m1,17 and G1m3 allotypes of trastuzumab. HLA-DR–associated peptides can originate from various regions of a protein typically occurring as multiple length variants that share the same HLA-DR binding core and form a “cluster." Clusters are indicated as black boxes, CDRs are indicated as shaded areas along the sequence of heavy chain (left) and light chain (right). Allotypic variations are indicated as vertical lines in the heavy chain. Clusters that were also detected in the media control samples are indicated as gray boxes. The detection of these peptides in the control samples can be explained by the use of human serum containing human IgG in the PBMC freezing media.