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. 2015 Dec 14;8(2):306–317. doi: 10.1080/19420862.2015.1116659

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Instrument configuration schematic. (A) A column manager housing 2 6-port 2-position valves was configured as illustrated by the schematic to facilitate transfer of retained drug species between the SPE (1^st) and RPLC (2^nd) dimensions. Valve position is denoted numerically as position 1 and 2. Abbreviations are defined as QSM: quaternary solvent manager, AS: auto sampler, TUV: tunable ultraviolet detector, BSM: binary solvent manager, MS: mass spectrometer, ACD: at-column-dilution. (B) Extracted drug species were transferred in a 5.50 min elution window using at-column-dilution with both valves in position 2 to refocus eluting drug species at the head of the analytical column. The transfer was bracketed with a 0.6 second interval in position 2,1 to purge the fluidic path.