Table 2.

2DLC recovery evaluation. Nac-linker-DSEA reference standard was prepared at 4 concentrations throughout the experimentally determined dynamic range and evaluate for recovery efficiency using the 2DLC configuration illustrated in Figure 4A. Identical separations were performed in a 1DLC configuration using the same system with a union in lieu of the SPE column (1^st dimension) as a reference. Comparison of peak area across triplicate injections of the NAc-linker-DSEA reference standard indicate sample recovery was equivalent between both 1DLC and 2DLC configurations.

Conc. (ng/mL)	1DLC			2DLC
	Area	R.SD (%)	R.E. (%)	Area	R.SD (%)	R.E. (%)
2	458	5.2	95.4	482	1.7	100.4
17	4117	0.2	99.6	4146	1.0	100.3
74	17776	1.7	99.2	18063	2.7	100.8
146	35556	1.9	101.0	34839	0.2	99.0